The exploration of hydrogen production promoting mechanism of Rhodobacter sphaeroides by expressing tetA under tetracycline stress |
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| Affiliation: | 1. Xi''an Key Laboratory of Sustainable Energy Materials Chemistry, Department of Applied Chemistry, School of Chemistry, Xi''an Jiaotong University, Xi''an 710049, China;2. State Key Laboratory of Multiphase Flow in Power Engineering, Department of Environmental Science and Engineering, School of Energy and Power Engineering, Xi''an Jiaotong University, Xi''an 710049, China;3. College of Eco-environmental Engineering, Qinghai University, Xi''ning 810016, China;1. Institute of Refrigeration and Cryogenic Engineering, Xi''an Jiaotong University, Xi''an 710049, China;2. China Special Equipment Inspection and Research Institute, Beijing, 100029, China;3. College of Petroleum and Chemical, Lanzhou University of Technology, Lanzhou, 730050, China;1. Indo-Korea Science and Technology Center (IKST), Jakkur, Bengaluru 560065, India;2. Electronic Materials Research Center, KIST, Seoul 136-791, South Korea;3. Center for Energy Materials Research, Korea Institute of Science and Technology, Seoul 02792, Seoul, Republic of Korea;1. Guangdong Energy Group Science and Technology Research Institute Co. Ltd., Guangzhou 510000, China;2. Guangdong Huizhou Lng Power Co. Ltd., Huizhou 516000, China;3. Guangdong Energy Group Co., Ltd., Guangzhou 510000, China;4. School of Materials and Physics, China University of Mining and Technology, Xuzhou 221116, China;1. Department of Metallurgical and Materials Engineering, Nevsehir Haci Bektas Veli University, Nevsehir, 50300, Turkey;2. Department of Metallurgical and Materials Engineering, Eskisehir Osmangazi University, Eskisehir, 26040, Turkey |
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| Abstract: | Photo-fermentative hydrogen production rate improving has been a key limiting link in the field of biological hydrogen production by photosynthetic bacterium (PSB) in recent years. In our previous study, apparent hydrogen production enhancement was observed in R. sphaeroides HY01 with tetA expressed under tetracycline (Tc), which attracted our interests. It was found that Tc-resistant strains always presented stable hydrogen production promotion on the maximum hydrogen production rate (Rm), which was approximately 30% with Tc addition. Figuring out the underlying connections between Tc resistance and hydrogen production of R. sphaeroides may provide new methods for obtaining engineered PSB with high hydrogen production performance. In this study, basing on experimental tests, hydrogen promotion of resistant strain was found to be Tc dose-dependent. Some relevant gene expression differences on hydrogen production of Rodobacter sphaeroides induced by the expression of TetA and the addition of Tc were also tested and discussed. |
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| Keywords: | Tetracycline Hydrogen production Divalent metal ions Gene expression difference |
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