Synergistic strategy for the enhancement of biohydrogen production from molasses through coculture of Lactobacillus brevis and Clostridium saccharobutylicum |
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| Affiliation: | 1. Botany and Microbiology Department, Faculty of Science, Suez University, Suez, 43518, Egypt;2. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt;3. Biomass Energy Engineering Research Centre, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, PR China;4. College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing, Zhejiang Province, 314001, PR China;1. School of Transportation and Vehicle Engineering, Shandong University of Technology, Zibo, 255000, China;2. State Key Laboratory of Engines, Tianjin University, Tianjin, 3000072, China;1. Institute of Modern Physics, Fudan University, Shanghai, 200433, China;2. Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, 621900, China;1. Biomass Energy Engineering Research Centre, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China;2. Shanghai Yangtze River Delta Eco-environmental Change and Management Observation and Research Station, Ministry of Science and Technology, 800 Dongchuan Road, Shanghai 200240, PR China;3. College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing, Zhejiang Province 314001, PR China;4. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt;5. Botany and Microbiology Department, Faculty of Science, Suez University, Suez 43721, Egypt;1. School of Materials Science and Engineering, Institute of New Energy Material Chemistry, Nankai University, Tianjin, 300350, PR China;2. College of Electronics Information and Optical Engineering, Nankai University, Tianjin, 300350, PR China;3. Computational Science Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea;1. Research Center for Combustion and Environment Technology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China;2. School of Electrical and Energy Power Engineering, Yangzhou University, Yangzhou, 225002, China;3. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China |
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| Abstract: | This study aimed to evaluate the capacity of different inoculum sources and their bacterial diversity to generate hydrogen (H2). The highest Simpson (0.7901) and Shannon (1.581) diversity indexes for H2-producing bacterial isolates were estimated for sewage inocula. The maximum cumulative H2 production (Hmax) was 639.6 ± 5.49 mL/L recorded for the sewage inoculum (SS30) after 72 h. The highest H2-producing isolates were recovered from SS30 and identified as Clostridium saccharobutylicum MH206 and Lactobacillus brevis MH223. The Hmax of C. saccharobutylicum, L. brevis, and synergistic coculture was 415.00 ± 24.68, 491.67 ± 15.90, and 617.67 ± 3.93 mL/L, respectively. The optimization process showed that the Hmax (1571.66 ± 33.71 mL/L) with a production rate of 58.02 mL/L/h and lag phase of 19.33 h was achieved by the synergistic coculture grown on 3% molasses at 40 °C, pH 7, and an inoculum size of 25% (v/v). This study revealed the economic feasibility of the synergistic effects of coculture on waste management and biohydrogen production technology. |
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| Keywords: | Anaerobic fermentation Bacterial cluster analysis Sewage sludge Biohydrogen Modified Gompertz model |
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