Feasibility of successive hydrogen and methane production in a single-reactor configuration of batch anaerobic digestion through bioaugmentation and stimulation of hydrogenase activity and direct interspecies electron transfer |
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| Affiliation: | 1. Winogradsky Institute of Microbiology, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences Leninskiy Pr-t, 33, 2, 119071, Moscow, Russia;2. Federal Scientific Agroengineering Center VIM 1st Institutsky Proezd, 5, 109428, Moscow, Russia;3. Faculty of Biology, Lomonosov Moscow State University Leninskie Gory, 1 b. 12, 119234, Moscow, Russia;1. State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China;2. School of Engineering Science, University of Chinese Academy of Sciences, Beijing, 100049, China;3. The University of Hong Kong, Hong Kong, 999077, China;4. School of Earth and Space Sciences, Peking University, Beijing, 100871, China;1. College of Resources and Environment, Chengdu University of Information Technology, Chengdu, Sichuan, 610225, China;2. School of Energy and Power Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China;3. Department of Mechanical Engineering, University of Kassala, Kassala, Sudan;1. School of Chemistry and Chemical Engineering, China University of Mining and Technology, 1 Daxue Street, Xuzhou, 221116, Jiangsu Province, China;2. School of Energy and Power Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, 210094, Jiangsu Province, China;1. Department of Chemical Engineering, University of Engineering and Technology, Lahore, Pakistan;2. Corporate Sustainability and Digital Chemical Management Department, Interloop Limited, Pakistan;1. Biomass Energy Engineering Research Centre, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China;2. Shanghai Yangtze River Delta Eco-environmental Change and Management Observation and Research Station, Ministry of Science and Technology, 800 Dongchuan Road, Shanghai 200240, PR China;3. College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing, Zhejiang Province 314001, PR China;4. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt;5. Botany and Microbiology Department, Faculty of Science, Suez University, Suez 43721, Egypt;1. Federal Scientific Agroengineering Center VIM, 1st Institutsky Proezd, 5, 109428, Moscow, Russia;2. Russian University of Transport, 127994, Moscow, Russia;3. Winogradsky Institute of Microbiology, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences Leninskiy Pr-t, 33, 2, 119071, Moscow, Russia;4. Malaviya National Institute of Technology Jaipur Jawaharlal Nehru Marg, Jaipur-302 017, Rajasthan, India |
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| Abstract: | Two-stage anaerobic digestion is a promising way to increase the efficiency of decomposition of organic waste. However, spatial separation of stages makes this technology less economically attractive. In this work, we studied a model of two-stage anaerobic digestion with stages separated in time in one reactor. To implement this model, we used a combination of (1) bioaugmentation with a hydrogen-producing Thermoanaerobacterium thermosaccharolyticum SP-H2, resistant to low pH, (2) setting a low initial pH (5.5) to reduce the activity of hydrogenotrophic methanogens, (3) supplementing additives in the form of soluble iron (II) and granular activated carbon (GAC) to stimulate hydrogenase activity and direct interspecies electron transfer (DIET), respectively. For comparison, two methanogenic inoculums with different dominant microbial groups and initial iron concentrations were used. With the simultaneous addition of GAC and iron (II), sequential production of hydrogen and methane was observed for both methanogenic inoculums. Without the addition of GAC, methane formation was practically not observed, which, apparently, indicated the impossibility of syntrophic degradation of acidogenesis products due to the high partial pressure of hydrogen and the absence of conductive material for activation of DIET. Significant differences in hydrogenase activity and the kinetics of hydrogen and methane formation according to the modified Gompertz equation depending on the iron content in the system may indicate the importance of this additive. Further studies should be aimed at determining the optimal initial pH, concentrations of stimulating additives, bioaugmentation and methanogenic cultures, as well as confirming the stability of the proposed single-reactor two-stage anaerobic digestion system in semi-continuous mode. |
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| Keywords: | Biohydrogen Biomethane Batch anaerobic digestion Direct interspecies electron transfer Hydrogenase activity |
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