不同基质奶粉中泛酸检测方法对比

目前,市面上水解蛋白奶粉的需求与日俱增,但是针对这类奶粉HPLC法分离效果较差,目标峰与杂峰不易分离,为解决这一问题,实验室从优化色谱条件、更换色谱柱、沉淀蛋白PH值调整等几个方面进行优化,同时采用微生物法与仪器法进行比对验证,为进一步解决部分水解蛋白奶粉泛酸不易检测的问题提供实验数据和研究材料。实验结果表明：微生物法和HPLC法检测一致性较好,精密度、准确性等均符合要求。微生物法检测范围广,市面上常见的婴幼儿配方奶粉均可检测;HPLC法适用于检测不含水解蛋白奶粉;调整流动相比例可以实现目标锋和杂峰的分离,但是耗时长、无规律、重复性低。

Comparison of detection methods of pantothenic acid in different matrix milk powder

Objective To compare the detection methods of pantothenic acid in different matrix milk powder, in order to explore the optimal method and conditions for the detection of pantothenic acid in different milk powder matrix. Methods Milk powder without hydrolyzed protein and milk powder with hydrolyzed protein were taken as research object. High performance liquid chromatography (HPLC) and microbiological method in GB 5009.210-2016 National food safety standard-Determination of pantothenic acid were used for detection. The advantages and disadvantages of the 2 methods were evaluated, and the optimal detection method was selected. Results For high performance liquid chromatograph, adjustment from the optimization of chromatographic conditions, replacement of chromatographic column, precipitation of protein, pH value and other aspects could not reduce the interference of miscellaneous peaks on the target peak. Only adjusting the proportion of mobile phase could achieve the separation of target peak and impurity peak. However, this method was time-consuming and irregular, would cause partial waste of equipment, consumables and personnel to a certain extent, and the repetition rate was low, so it was not suitable to use. The accuracy and stability of microbiological method for different matrix milk powder (including hydrolyzed protein milk powder) could meet the requirements of the method. Conclusion For most milk powder (except hydrolyzed protein matrix), high performance liquid chromatography and microbiological method have good consistency, the precision and accuracy meet the requirements; For hydrolyzed protein milk powder, the accuracy and stability of microbiological method are better than those of high performance liquid chromatography.