| 免疫毒素EGFR mab-RTA的制备及鉴定 |
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| 引用本文： | 申苏建,吴金明,金思思,黄智铭,吴建胜.免疫毒素EGFR mab-RTA的制备及鉴定[J].粉末涂料与涂装,2010,23(5). |
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| 作者姓名： | 申苏建 吴金明 金思思 黄智铭 吴建胜 |
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| 作者单位： | 温州医学院附属第一医院消化内科,浙江温州,325000 |
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| 摘 要： | 目的制备由抗表皮生长因子受体(EGFR)单克隆抗体(mab)与蓖麻毒蛋白A链(RTA)偶联而成的免疫毒素(IT),并进行鉴定。方法以N-琥珀酰胺-3(-2-吡啶二硫)丙酸酯(SPDP)为偶联剂,制备免疫毒素EGFRmab-RTA,SDS-PAGE分析其纯度及成分,DAB显色法检测其对人肝癌HepG2细胞的结合能力。结果 SDS-PAGE分析显示免疫毒素成功构建,偶联物中EGFRmab与RTA的偶联比为1:1.16,免疫毒素基本保留了EGFRmab对HepG2细胞的结合能力。结论利用SPDP可实现EGFRmab与RTA的成功偶联。
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| 关 键 词： | 表皮生长因子受体 单克隆抗体 蓖麻毒蛋白A链 免疫毒素 |
Preparation and Identification of Immunotoxin EGFR mab-RTA |
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| SHEN Su-jian,WU Jin-ming,JIN Si-si,et al.Preparation and Identification of Immunotoxin EGFR mab-RTA[J].Chinese Journal of Biologicals,2010,23(5). |
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| Authors: | SHEN Su-jian WU Jin-ming JIN Si-si |
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| Affiliation: | SHEN Su-jian,WU Jin-ming,JIN Si-si,et al(Department of Gastroenterology,The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,Zhejiang Province,China) |
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| Abstract: | Objective To prepare an immunotoxin(IT)by coupling the monoclonal antibody(mab)against epidermal growth factor receptor(EGFR)to ricin A(RTA),then identify.Methods IT EGFR mab-RTA was prepared using N-succinimidyl-3(-2pyridyldithio)-propionate(SPDP)as a coupler,then analyzed for purity and component by SDS-PAGE,and determined for binding ability to human hepatocarcinoma HepG2 cells by DAB coloration.Results SDS-PAGE showed that IT EGFR mab-RTA was successfully constructed,in which the coupling ratio of EGFR ... |
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| Keywords: | Epidermal growth factor receptor(EGFR) Monoclonal antibody(mab) Ricin A(RTA) Immune toxin |
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