肺癌组织中cPLA2和mPGES-1的表达及意义

目的探讨胞浆型磷脂酶A2（cPLA2）、微粒体前列腺素E合成酶-1（mPGES-1）在肺癌组织中的表达及临床意义,为肺癌发生发展的分子机制提供理论依据。方法采用RT-PCR检测60例肺癌（肺癌组）和癌旁（癌旁组）组织中cPLA2、mPGEs-1mRNA表达水平,采用免疫组织化学SP法检测2组组织中cPLA2、mPGEs-1蛋白表达水平。结果肺癌组组织中cPLA2、mPGES-1mRNA表达水平均明显高于癌旁组（均P〈0.05）。肺癌组组织中cPLA2蛋白阳性表达率为76.7%（46/60）,癌旁组组织中cPLA2蛋白无阳性表达,2组比较差异有统计学意义（P〈0.05）;肺癌组组织中mPGES-1蛋白阳性表达率为66.7%（40/60）,明显高于癌旁组的10.0%（6/60）（P〈0.05）。肺癌组的肿瘤〈5cm、组织学类型为鳞癌、病理分级为高中分化、淋巴结无转移和TNM分期为Ⅰ期cPLA2蛋白阳性表达率与肿瘤≥5cm、组织学类型为腺癌、病理分级为低分化、淋巴结有转移和TNM分期为Ⅱ、Ⅲ期比较差异均无统计学意义（均P〉0.05）;肺癌组的肿瘤〈5cm、组织学类型为鳞癌mPGEs-1蛋白阳性表达率与肿瘤≥5cm、组织学类型为腺癌比较差异均无统计学意义（均P〉0.05）,肺癌组的病理分级为高中分化、淋巴结无转移和TNM分期为Ⅰ期mPGEs-1蛋白阳性表达率均明显低于病理分级为低分化、淋巴结有转移和TNM分期为Ⅱ、Ⅲ期（均P〈0.05）。结论 cPLA2、mPGES-1的高表达可能在肺癌的发生发展中起着重要作用,其可能为肺癌的早期诊断和为开发肺癌的靶向治疗提供一定的临床依据。

Expression and Significance of cPLA2 and mPGES-1 in Lung Cancer

Objective To explore the expression and clinical significance of cytosolic phospholipase A2（cPLA2） and microsomal prostaglandin E synthase-1 （mPGES-1） in lung cancer,and to provide a theoretical basis for the molecular mechanism of the development of lung cancer. Methods The mRNA and protein expression of cPLA2 and mPGES-1 in cancer tissues and adjacent tissues was detected by RT-PCR and immunohistochemical method in 60 cases of lung cancer,re- spectively. Results The mRNA expression of cPLA2 and mPGES-1 in cancer tissues was signifi- cantly higher than that in adjacent tissues（P〈0.05）. Furthermore, the positive expression rates of cPLA2 and mPGES-1 in cancer tissues were significantly higher than those in adjacent tissues （76.7% vs 0.0% and 66.7% vs 10.0%,respectively;P〈0.05）. There were no significant differences in the positive rate of cPLA2 protein expression between squamous cell carcinoma（tumor size〈5 cm,well differentiation,TNM stage Ⅰ,and no lymph node metastasis） and adenocarcinoma （tumor size≥ 5 cm, poor differentiation, TNM stage Ⅱ-Ⅲ, and lymph node metastasis）（P〈 0.05）. In addition,there were no significant differences in the positive rate of mPGEsprotein ex- pression between squamous cell carcinoma with tumor size〈5 cm and adenocarcinoma with tumor size〈5 cm（P〈0.05）. However, the positive rate of mPGEsprotein expression in well-differenti ated stage I carcinoma without lymph node metastasis was significantly lower than that in poordifferentiated stage Ⅱ-Ⅲ carcinoma with lymph node metastasis（P〈0.05）. Conclusion The high expression of cPLA2 and mPGES-1 may play an important role in the occurrence and development of lung cancer. The detection of cPLA2 and mPGES-1 expression may provide a clinical basis for the early diagnosis and targeted therapy of lung cancer.