| 盐酸羟胺和紫外线复合诱变选育纳豆激酶高产菌株 |
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| 引用本文： | 关志炜,李志香,孙洪涛.盐酸羟胺和紫外线复合诱变选育纳豆激酶高产菌株[J].食品与发酵工业,2009,35(6). |
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| 作者姓名： | 关志炜 李志香 孙洪涛 |
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| 作者单位： | 1. 山东教育学院,生物科学与技术系,山东,济南,250013
2. 山东福瑞达生物工程有限公司,山东,济南,250014 |
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| 摘 要： | 以从日本纳豆中分离出的1株枯草芽孢杆菌纳豆亚种(Bacillus subtlis natto,简称纳豆菌)NT-6为出发菌株,通过盐酸羟胺和紫外线复合诱变处理,采用利福平抗性平板筛选和发酵筛选相结合的方法,获得了1株突变株NT207,其产酶能力比诱变前提高了68%,在未经优化的液态发酵条件下,纳豆激酶的酶活力达到了648.72IU/mL。
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| 关 键 词： | 纳豆激酶 菌种选育 酶活力 复合诱变 |
Research on the Breeding of Bacillus subtilis Natto Highly Producing Nattokinase by the Compound Mutation of Hydroxylamine Hydrochloride and UV Radiation |
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| Guan Zhiwei,Li Zhixiang,Sun Hongtao.Research on the Breeding of Bacillus subtilis Natto Highly Producing Nattokinase by the Compound Mutation of Hydroxylamine Hydrochloride and UV Radiation[J].Food and Fermentation Industries,2009,35(6). |
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| Authors: | Guan Zhiwei Li Zhixiang Sun Hongtao |
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| Abstract: | Bacillus subtilis natto NT-6 strain was isolated from a kind of Japanese functional food. In order to en-hance the nattokinase-producing ability of NT-6, the compound mutation of hydroxylamine hydrochloride and UV radi-ation was performed. After the compound mutation, the mutant strain NT207 was obtained by the screening of anti-ri-fampin plate and then liquid fermentation. NT207's ability of producing nattokinase was more remarkable than the strain NT-6 by 68%. The activity of nattokinase produced by the strain NT207 in the condition of the liquid fermenta-tion medium that hasn't been optimized can reach to 648.72 IU/mL. |
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| Keywords: | nattokinase breeding of strains enzyme activity compound mutation |
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