Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation |
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Authors: | Liao Wang Yisheng Chen Zhiyang Ye Bernhard Hellmann Xueming Xu Zhengyu Jin Qinqin Ma Na Yang Fengfeng Wu Yamei Jin |
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Affiliation: | 1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,China;2.School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Lab for Cereal Fermentation Technology,Jiangnan University,Wuxi,China;3.Institute of Nutritional Science, and Interdisciplinary Research Center,Justus Liebig University Giessen,Giessen,Germany;4.College of Life Science,Sichuan Normal University,Chengdu,China |
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Abstract: | The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v/v/v) to 70 mm solvent front, the plate was subjected to a standardized 1,1-diphenyl-2-picrylhydrazyl assay. In addition to synoptical eye inspection, accurate quantification was realized by modified densitometric measurements: fluorescence mode, excitation wavelength 530 nm (D2 and W lamp) without optical filter, which offered satisfactory sensitivity (8.5–17.5 mg/kg) and acceptable linearity (R2?>?0.999 within 50–200 ng/zone). Moreover, the established method was validated with edible oil samples, against EU Directive 2006/52/EC. Apart from that, the unambiguous confirmation of positive results was conveniently achieved by TLC-MS interface-mediated mass spectrometry. Featuring the merits of screening conception, the proposed method not only reached the goal of accurate quantification and conclusive identification of multi-phenolic antioxidants, but also excellently balanced the simplicity, detectability, and throughput of the screening workup. Therefore, it might be an attractive alternative to conventional methods. |
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