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| 反相高效液相色谱法测定甘油二酯含量研究 | |
| 引用本文： | 汪勇,宋坷珂,王丽丽,韩雪,赵谋明. 反相高效液相色谱法测定甘油二酯含量研究[J]. 中国粮油学报, 2010, 2012(3) |
| 作者姓名： | 汪勇 宋坷珂 王丽丽 韩雪 赵谋明 |
| 作者单位： | 1. 华南理工大学轻工与食品学院,广州,510641;暨南大学食品科学与工程系,广州,510632 2. 暨南大学食品科学与工程系,广州,510632 3. 华南理工大学轻工与食品学院,广州,510641 |
| 基金项目： | 广东省科技攻关重点项目 |
| 摘 要： | 用分子蒸馏和柱层析的方法制备了大豆油甘油二酯(DAG)的标准样品,通过高效液相-电喷雾质谱联用(HPLC-ESI-MS)和薄层色谱法(TLC)验证了标准样品的纯度。用反相高效液相色谱(RP-HPLC)在UV=210 nm做大豆油DAG的标准曲线,在质量浓度范围1.0~12.0 mg/mL,大豆油DAG标准曲线线性良好。通过加样回收试验表明,加样回收率在104%~109%之间,RSD在0.69%~5.19%之间。并用该方法分析了大豆油、分子蒸馏DAG样品中的DAG。 |
| 关 键 词： | 甘油二酯 反相高效液相色谱 分子蒸馏 |
Determination of Diacylglycerol Content by Reversed Phase High Performance Liquid Chromatography |
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| Wang Yong,Song Keke Wang Lili Han Xue Zhao Mouming(College of Light industry,Food Science,South China University of Technology,Guangzhou. Determination of Diacylglycerol Content by Reversed Phase High Performance Liquid Chromatography[J]. Journal of the Chinese Cereals and Oils Association, 2010, 2012(3) | |
| Authors: | Wang Yong Song Keke Wang Lili Han Xue Zhao Mouming(College of Light industry Food Science South China University of Technology Guangzhou |
| Affiliation: | Wang Yong1,2 Song Keke2 Wang Lili2 Han Xue2 Zhao Mouming1(College of Light industry , Food Science,South China University of Technology1,Guangzhou 510641)(Department of Food Science , Engineering,Jinan University2,Guangzhou 510632) |
| Abstract: | Soybean diacylglycerols(DAG) reference material was prepared by combination of molecular distillation and column chromatography.The purity of the reference material was verified by high performance liquid chromatography /electrospray ionization /mass spectrometry and thin layer chromatography.Results:The calibration curve of soybean DAG built by reversed phase-HPLC at UV=210 nm shows a good linear at concentrations in range of 1.0~12.0 mg/mL.Recovery experiments show the recoveries of samples are in range o... |
| Keywords: | diacylglycerol reversed phase-HPLC molecular distillation |
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