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酸奶中污染酵母菌和霉菌的分离及鉴定
引用本文:赵泰霞,孟祥晨,张巧云.酸奶中污染酵母菌和霉菌的分离及鉴定[J].食品工业科技,2012(2):189-192.
作者姓名:赵泰霞  孟祥晨  张巧云
作者单位:东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨,150030
摘    要:以室温放置直至变质的市售酸奶为样品,从中分离出2株酵母菌和4株霉菌,分别命名为Y1、Y2、M1、M2、M3、M4。酵母菌采用形态学和26SrDNAD1/D2区序列分析鉴定,确定Y1为乳酸克鲁维酵母(Kluyveromyces lactis),Y2为马克思克鲁维酵母(Kluyveromyces marxianus)。霉菌经菌落及菌体形态学鉴定,确定M1、M2、M4为青霉(Penicillum),M3为白地霉(Geotrichum candidum)。

关 键 词:酸奶  酵母菌  霉菌  分离  鉴定

The isolation and identification of yeast and molds from the spoilage yogurts
ZHAO Tai-xia,MENG Xiang-chen,ZHANG Qiao-yun.The isolation and identification of yeast and molds from the spoilage yogurts[J].Science and Technology of Food Industry,2012(2):189-192.
Authors:ZHAO Tai-xia  MENG Xiang-chen  ZHANG Qiao-yun
Affiliation:(Key Laboratory of Dairy Science,Ministry of Education,Food Science and Technology College,Northeast Agricultural University,Harbin 150030,China)
Abstract:Two yeast strains(marked Y1,Y2)and 4 molds strains(marked M1,M2,M3,M4)were isolated from spoilage yogurts which were purchased from local market.The yeast strains were identified by observing its morphology and 26S rDNA D1/D2 region sequence analysis,the results showed that Y1 was Kluyveromyces lactis,Y2 was Kluyveromyces marxianus.The molds strains were identified by colony and cell morphology,and M1,M2,M4.were Penicillum,M3 was Geotrichum candidum.
Keywords:yogurt  yeast  mold  isolation  identification
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