| 一步提取测定食品中的脂肪酸 |
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| 引用本文： | 黄菲菲,赵嘉胤,王建军,陈景春.一步提取测定食品中的脂肪酸[J].食品工业科技,2012(2):77-79. |
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| 作者姓名： | 黄菲菲 赵嘉胤 王建军 陈景春 |
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| 作者单位： | 农业部食品质量监督检验测试中心（上海）,上海,200436 |
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| 摘 要： | 建立了一步提取测定食品中脂肪酸的检测方法。乙酰氯与甲醇反应得到的盐酸-甲醇使试样中的脂肪和游离脂肪酸甲酯化,用甲苯提取后,氢火焰离子化检测器检测。各脂肪酸甲酯标准溶液在0.005～1mg/mL范围内呈线性相关(r=0.999);在不同基质中各脂肪酸回收率为82.1%～100%,相对标准偏差RSD<10%;各脂肪酸检出限为1mg/100g。一步提取测定脂肪酸操作方便、快速,灵密度高,可满足食品中脂肪酸测定的要求。
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| 关 键 词： | 脂肪酸 气相色谱 一步提取 食品 |
Determination of fatty acids in foods by one-step extraction |
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| HUANG Fei-fei,ZHAO Jia-yin,WANG Jian-jun,CHEN Jing-chun.Determination of fatty acids in foods by one-step extraction[J].Science and Technology of Food Industry,2012(2):77-79. |
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| Authors: | HUANG Fei-fei ZHAO Jia-yin WANG Jian-jun CHEN Jing-chun |
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| Affiliation: | (Food Quality Supervision and Testing Center of Ministry of Agriculture,Shanghai 200436,China) |
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| Abstract: | A method of one-step extraction determination of fatty acids in foods by gas chromatography(GC)was developed.Samples were esterified by hydrochloric acid-methanol,which was the reaction product of acetyl chloride and methanol.Extracting with toluene,contents of fatty acids were determined by GC with FID detector.The method had a good linear relativity(r=0.999)between 0.005~1mg/mL.Recovery rate in different samples range from 82.1% to 100%,RSD<10%,with the LOD of 1mg/100g.With the advantages of easy-handling,fast and high sensitivity,one-step extraction method could meet the requirement of the determination of fatty acids in foods. |
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| Keywords: | fatty acids GC one-step extraction foods |
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