小麦胚芽中黄酮类物质的超临界CO2萃取及其抗氧化作用

研究了超临界流体萃取方法的工艺条件对小麦胚芽中黄酮类物质的提取率的影响,并探讨了麦胚黄酮粗提物对菜籽油过氧化的抑制作用及与其他物质的协同作用。实验结果表明,超临界流体萃取小麦胚芽中黄酮类物质的最佳工艺条件为萃取温度45℃、萃取压力30MPa、CO2流量25L/h、夹带剂无水乙醇的用量2mL/g。经紫外分光光度法分析,在此条件下,每千克小麦胚芽提取总黄酮类物质量达8.029g。麦胚黄酮粗提物具有很强的抗氧化活性,可有效延缓油脂脂质过氧化反应;其抗氧化作用优于常规醇提法的麦胚黄酮粗提物、TBHQ和槲皮素的抗氧化性能,与其添加量呈正相关性;VC和柠檬酸对麦胚黄酮粗提物有一定的协同作用,VC增效作用大于柠檬酸。

Research on the SFE CO2 conditions for flavonoids from wheat germ and its antioxidation

The influence of supercritical fluid extraction(SFE) process conditions on the yield of flavonoids substances from wheat germ,and the inhibition of wheat germ flavonoids crude extractive to colza oil peroxide,and the synergy of other substances were studied.The results showed that the optimum SFE technological conditions for flavonoids substances was temperature 45℃,pressure 30MPa,the rate of CO2 flow 25L/h,the entrainment agents(anhydrous alcohol) 2mL/g.Analyzed by uv spectrophotometry,the extraction quantity of total flavonoids achieves 8.029g from 1kg wheat germ.The flavonoids crude extractive with strong antioxidant activity could effectively relieve grease lipid peroxidation.Positively correlated to additive amount,the antioxidation was better than wheat germ flavonoids crude extractive by the traditional alcohol extraction and TBHQ and quercetin.VC and citric acid were synergetic to the flavonoids crude extractive.As for synergistic effect,VC was more than citric acid.