| 正交实验法优化葡萄藤茎中多酚活性物质提取工艺的研究 |
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| 引用本文： | 史红梅,孙玉霞,蒋锡龙,魏彦锋,王恒振.正交实验法优化葡萄藤茎中多酚活性物质提取工艺的研究[J].食品工业科技,2012(2):287-290. |
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| 作者姓名： | 史红梅 孙玉霞 蒋锡龙 魏彦锋 王恒振 |
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| 作者单位： | 山东省酿酒葡萄科学研究所,山东济南,250100 |
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| 基金项目： | 山东省科技攻关计划;葡萄酒产业科技特派员创业链示范建设(2009GG2ZC08013) |
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| 摘 要： | 对葡萄藤茎中多酚活性物质提取的最佳工艺条件进行了探讨。对影响提取液多酚含量的乙醇浓度、料液比、提取温度、提取时间进行单因素实验,在此基础上设计了4因素3水平的正交实验,以提取液中多酚含量和DPPH.清除率为指标进行实验。结果表明:多酚活性物质的最佳工艺条件为提取温度60℃,固液比1:15,乙醇浓度50%,提取时间1h。在此条件下提取液中多酚含量为14.43mg/g,DPPH.清除率81.23%。
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| 关 键 词： | 多酚 葡萄藤茎 DPPH· 正交实验 |
Orthogonal test as an approach to optimize the extraction of polyphenol from grapevine cane |
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| SHI Hong-mei,SUN Yu-xia,JIANG Xi-long,WEI Yan-feng,WANG Heng-zhen.Orthogonal test as an approach to optimize the extraction of polyphenol from grapevine cane[J].Science and Technology of Food Industry,2012(2):287-290. |
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| Authors: | SHI Hong-mei SUN Yu-xia JIANG Xi-long WEI Yan-feng WANG Heng-zhen |
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| Affiliation: | (Shandong Vine and Wine-Making Institute,Jinan 250100,China) |
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| Abstract: | The optimal extracting conditions of polyphenol from grapevine cane were studied.The single factor experiments of alcohol concentration,the ratio of solid to liquid,extracting temperature and extracting time were investigated.And 4 factors 3 levels orthogonal test was devised to obtain the optimal extracting conditions based on the single factor experiment.The optimum extracting process of polyphenols from the grapevine cane was listed as follows:extracting temperature 60℃,ratio of solid to liquid 1:15,alcohol concentration 50% and extracting time 1h.The yield of polyphenols was found to be 14.43mg/g and DPPH· clearance rate was 81.23%. |
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| Keywords: | polyphenol grape cane DPPH· orthogonal test |
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