| Hansenula anomala转化葡萄糖生产阿拉伯糖醇的研究 |
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| 引用本文： | 唐晓芳,张国栋,王刚.Hansenula anomala转化葡萄糖生产阿拉伯糖醇的研究[J].食品工业科技,2012(1):314-317. |
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| 作者姓名： | 唐晓芳 张国栋 王刚 |
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| 作者单位： | 1. 西华大学生物工程学院,四川成都,610039
2. 四川省食品发酵工业研究设计院,四川成都,611130 |
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| 摘 要： | 对Hansenula anomala转化葡萄糖生产D-阿拉伯糖醇的反应条件进行了研究。高供氧量有利于阿拉伯糖醇的生成。在一定范围内,葡萄糖初始浓度越高,阿拉伯糖醇的产量也随之明显提高。并通过正交实验,确定了最佳发酵条件为(g/L):葡萄糖500,酵母膏2,蛋白胨2,CaCl20.5,(NH4)2SO45,MgSO41,KH2PO43,pH5.0;发酵温度30℃,装液量15～20mL/500mL三角瓶,发酵时间263h。阿拉伯糖醇浓度为245.97g/L,转化率为0.49g阿拉伯糖醇/g葡萄糖,平均反应强度0.935g/Lh。
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| 关 键 词： | 异常汉逊氏酵母(Hansenula anomala) D-阿拉伯糖醇 发酵 |
Study on production of arabitol from glucose by Hansenula anomala |
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| TANG Xiao-fang,ZHANG Guo-dong,WANG Gang.Study on production of arabitol from glucose by Hansenula anomala[J].Science and Technology of Food Industry,2012(1):314-317. |
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| Authors: | TANG Xiao-fang ZHANG Guo-dong WANG Gang |
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| Affiliation: | 1.Department of Bioengineering,Xihua University,Chengdu 610039,China;2.Sichuan Provincial Food Fermentation Industry Research and Design Institute,Chengdu 611130,China) |
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| Abstract: | Production of D-arabitol from glucose by Hansenula anomala strain GA24 was studied.Some of the influencing factors were determined:aeration,osmotic pressure and medium.The best fermentation conditions were(g/L):glucose 500,yeast extract 2,peptone 2,CaCl2 0.5,(NH4)2SO4 5,MgSO4 1,KH2PO4 3,pH5.0;the temperature at 30℃,15～20mL medium contained in 500mL Erlenmeyer flask,and 263h of cultivation. |
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| Keywords: | Hansenula anomala D-Arabitol fermentation |
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