| 海带中岩藻黄质的C18-HPLC-PDA检测 |
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| 引用本文： | 宫平,陈菲,惠伯棣.海带中岩藻黄质的C18-HPLC-PDA检测[J].食品工业科技,2012(1):342-343,351. |
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| 作者姓名： | 宫平 陈菲 惠伯棣 |
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| 作者单位： | 北京联合大学应用文理学院,北京,100191 |
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| 基金项目： | 十一五国家科技支撑计划项目(2008BAI58B06) |
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| 摘 要： | 目的:研究海带中岩藻黄质的C18-HPLC-PDA检测方法。方法:在C18-HPLCPDA上,海带中的岩藻黄质可获得良好的分离。色谱条件:DiamonsilTM C18(4.6mm×250mm,5μm)柱;流动相A:乙腈-水(9∶1,V/V);流动相B:乙酸乙酯;线性梯度洗脱:B在20min内由0增至100%;流速:1.0mL/min;PDA波长范围:250～600nm;进样量:20μL。根据其色谱行为和光谱特征,使用外标法可对岩藻黄质组分定性、定量。
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| 关 键 词： | 岩藻黄质 海带 HPLC |
Determination of fucoxanthin from Laminaria japonica by C18-HPLC-PDA |
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| GONG Ping,CHEN Fei,HUI Bo-di.Determination of fucoxanthin from Laminaria japonica by C18-HPLC-PDA[J].Science and Technology of Food Industry,2012(1):342-343,351. |
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| Authors: | GONG Ping CHEN Fei HUI Bo-di |
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| Affiliation: | (College of Arts & Science,Beijing Union University,Beijing 100191,China) |
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| Abstract: | Objective:The fucoxanthin from Laminaria japonica was determined by C18-HPLC-PDA.Methods:By C18-HPLC-PDA,fucoxanthin from brown alga was readily separated under following conditions:DiamonsilTM C18(4.6mm×250mm,5μm);mobile phase A:acetonitrile-water(9:1,V/V);mobile phase B:ethyl acetate;linear gradient:B increased from 0 to 100% in 20min;flow rate:1.0mL/min;PDA wavelength range:250～600nm;sample injection volume:20μL.According to its chromatographic behavior and spectral characteristics,fucoxanthin was identified and quantified with the aid of an external standard. |
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| Keywords: | fucoxanthin seaweed HPLC |
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