| 应用DPO技术检测食源性致病菌的多重PCR的研究 |
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| 引用本文： | 卢富山,王云龙,景建洲. 应用DPO技术检测食源性致病菌的多重PCR的研究[J]. 食品工业科技, 2012, 0(2): 199-202 |
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| 作者姓名： | 卢富山 王云龙 景建洲 |
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| 作者单位： | 1. 郑州轻工业学院食品与生物工程学院,河南郑州,450002
2. 河南省生物工程技术研究中心,河南郑州,450002 |
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| 基金项目： | 河南省杰出青年基金项目(74100510007) |
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| 摘 要： | DPO是经特殊设计的特异性引物,本研究根据单增李斯特菌的prfA gene、O157的rfb Egene和副溶血弧菌的tlgene和志贺氏菌的ipaH gene设计四对DPO引物,采用多重PCR的方法,建立了检测单增李斯特菌、大肠杆菌O157:H7、副溶血弧菌和志贺氏菌的快速检测体系。实验结果表明:该体系特异性强,操作简单,为食源性致病菌的检测提供了新型的有效手段。
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| 关 键 词： | DPO引物 多重PCR 食源性致病菌 |
Multiplex-PCR for detection of foodborne pathogens with a dual priming oligonucleotide(DPO)system |
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| LU Fu-shan,WANG Yun-long,JING Jian-zhou. Multiplex-PCR for detection of foodborne pathogens with a dual priming oligonucleotide(DPO)system[J]. Science and Technology of Food Industry, 2012, 0(2): 199-202 |
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| Authors: | LU Fu-shan WANG Yun-long JING Jian-zhou |
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| Affiliation: | 1,*(1. Zhengzhou University of Light Industry,Zhengzhou 450002,China;2. Henan Bioengineering Technology Research Center,Zhengzhou 450002,China) |
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| Abstract: | DPO is one of the new specific primers.In this study,four sets of dual priming oligonucleotide(DPO)primers were selected according to Listeria monocytogenes prfA gene,E.coli O157:H7 rfbE gene,Vibrio parahaemolyticus tl gene,Shigella ipaH gene,respectively,and applied in the detection of the four previously mentioned foodborne pathogens with multiplex polymerase chain reaction(mPCR).The results showed that DPO-mPCR exhibited high PCR specificity even under less than optimal PCR condition compared with conventional mPCR.Meanwhile,this method is easy to operate. |
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| Keywords: | DPO primers mPCR foodborne pathogens |
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