| 茶树菇多糖的提取、气相色谱分析和AFM观测以及活性研究 |
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| 引用本文： | 窦佩娟,张静,孙润广,焦自明,李佳媚. 茶树菇多糖的提取、气相色谱分析和AFM观测以及活性研究[J]. 食品工业科技, 2012, 0(2): 93-96 |
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| 作者姓名： | 窦佩娟 张静 孙润广 焦自明 李佳媚 |
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| 作者单位： | 1. 陕西师范大学物理学与信息技术学院,生物物理与生物医学工程实验室,陕西西安710062
2. 陕西师范大学食品工程与营养科学学院,陕西西安,710062 |
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| 基金项目： | 国家自然科学基金(10874108);陕西省自然科学基础研究计划(SJ08A16) |
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| 摘 要： | 利用水提得到茶树菇水提多糖S-ACP,并对水提残渣进行碱提得多糖J-ACP。分别用考马斯亮蓝G-250法及苯酚硫酸法测定蛋白含量及总糖含量,气相色谱(GC)分析多糖组分,原子力显微镜(AFM)观测结构形貌,并进行了体外抗氧化活性测定。结果表明,S-ACP的单糖组成为木糖、甘露糖、葡萄糖、半乳糖,其摩尔比为2.527:0.140:1.284:1.738;J-ACP的单糖组成为鼠李糖、木糖、甘露糖、葡萄糖、半乳糖,其摩尔比为1.327:2.525:0.140:3.641:1.765;AFM观测表明S-ACP为多侧支的雪花状结构,J-ACP为少侧支的简单环状结构,且二者均非单链存在;抗氧化活性结果表明S-ACP活性高于J-ACP。
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| 关 键 词： | 茶树菇 多糖 气相色谱 原子力显微镜 |
Study on the extraction,GC analysis,AFM observation and activity of polysaccharide from Agrocybe aegerita |
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| DOU Pei-juan,ZHANG Jing,SUN Run-guang,JIAO Zi-ming,LI Jia-mei. Study on the extraction,GC analysis,AFM observation and activity of polysaccharide from Agrocybe aegerita[J]. Science and Technology of Food Industry, 2012, 0(2): 93-96 |
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| Authors: | DOU Pei-juan ZHANG Jing SUN Run-guang JIAO Zi-ming LI Jia-mei |
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| Affiliation: | 1(1. Laboratory of Biophysics and Biomedical Technology,College of Physics and Information Technology,Shaanxi Normal University,Xi’an 710062,China;2. College of Food Engineering and Nutrition Science,Shaanxi Normal University,Xi’an 710062,China) |
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| Abstract: | A polysaccharide named S-ACP was extracted from Agrocybe aegerita by hot water,then a polysaccharide named J-ACP was isolated from the dried leaf mustard with alkali.The content of the protein and the total sugars were determined by Coomassic brilliant blue G-250 method and phenol-sulfuric acid method.The monosaccharide compositions of polysaccharides were analyzed by gas chromatography(GC).The structure of polysaccharides were investigated by atomic force microscopy(AFM).Meanwhile,the antioxidant activity in vitro of polysaccharides were also tested.Conclusion:Xyl,Man,Glu and Gal were determined to be the components of S-ACP in the mol ratios of 2.527:0.140:1.284:1.738.Rha,Xyl,Man,Glu and Gal were determined to be the components of J-ACP in the mol ratios of 1.327:2.525:0.140:3.641:1.765.It was found that S-ACP on mica formed Multi-lateral Snowflake-like structure by AFM,while J-ACP existed in the form of simple ring-like structure.The results showed that S-ACP had stronger antioxidant activity than J-ACP. |
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| Keywords: | Agrocybe aegerita polysaccharide gas chromatography atomic force microscopy |
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