Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples |
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Authors: | M Maher M Glennon G Martinazzo E Turchetti S Marcolini T Smith MT Dawson |
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Affiliation: | BioResearch Ireland, National Diagnostics Centre, University College Galway, Ireland. |
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Abstract: | We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay. |
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