基于城市污水资源化的跑道池培养小球藻条件优化

利用城市污水培养微藻，可在实现污水无害化处理的同时，培养微藻回收生物质能源。鉴于跑道池与污水处理厂的氧化沟在原理和结构上都具有许多相似之处，将氧化沟改造成用于微藻培养的跑道池，可以实现污水处理与微藻生物柴油生产的耦合。本文主要研究了藻种对温度和pH的适应性，并考察了小球藻在跑道池培养系统中的实际应用效果，探讨了利用城市污水连续培养小球藻工艺应用的可行性。结果表明：温度在20~40℃区间，蛋白核小球藻诱变株都能生长，小球藻可承受的pH范围为5.7~9.7。利用跑道池系统可以实现污水处理与微藻培养的耦合，蛋白核小球藻最高干重为0.2867g/L，最高油脂产量可达0.0696g/L，出水水质稳定达到国家排放标准。随着培养规模的扩大，跑道池培养工艺具有良好的应用前景。

Optimization of culture condition for biodiesel production by Chlorella pyrenoidosa grown in municipal wastewater in open raceway ponds

Cultivating microalgae with municipal wastewater can achieve not only treatment of waste water,but also recovery algal biofuel as energy source. Considering the similarities in principle and structure between raceway pond and oxidation ditch,this study aimed at turning the oxidation ditch into raceway pond,coupling of wastewater treatment and microalgae cultivation. This study investigated the adaptability of C. pyrenoidosa to different temperatures and pHs,and discussed the feasibility of coupling wastewater treatment and microalgae cultivation using raceway pond system. The results showed that the C. pyrenoidosa mutant strain could bear with temperature between 20℃ and 40℃,and pH at the range of 5.7-9.7. Coupling wastewater treatment and microalga cultivation using the raceway pond system was feasible. The highest dry weight of C. pyrenoidosa was 0.2867 g/L,and the highest lipid yield of was up to 0.0696g/L. The water quality of effluent could consistenly meet the national emission standards. With the expansion of cultivation scale,open-pond culture technology had a great application prospect.