真菌发酵酒精废弃物合成单细胞蛋白基质及菌种筛选

为了优选利用酒精废弃物生产单细胞蛋白（single cell protein，SCP）的最佳基质和菌种组合，从而实现其高值化利用，本文选取了黄酒加工废弃物（黄酒糟和米浆水）以及木薯酒精加工废弃物（木薯原糟和木薯酒糟上清液）4种基质作为发酵底物，并以产朊假丝酵母（Candida utilis）和白地霉（Geotrichum candidum）2种常见菌种作为筛选对象，通过摇瓶发酵实验，考察不同基质对菌种产SCP的影响，同时对比不同菌种利用酒精废弃物发酵合成SCP的能力，从而选出最优发酵基质和菌株。结果表明，以黄酒糟和米浆水按质量分数6%配比，同时接种产朊假丝酵母和白地霉进行混菌发酵4天时，SCP的产率及产量均达最优值，干物质产率可达3.91，产量可达90.22g/L，粗蛋白含量可达53.3%，且发酵产物中的氨基酸成分齐全，作为蛋白饲料具有良好的应用前景。

Screening of substrates and strains for producing single cell protein by fungal fermentation in distillery waste

To utilize distillery residue to produce value-added products, the productions of single cell protein (single cell protein, SCP)from various substrates and strains were investigated. Yellow winery residue (yellow wine lees and rice wastewater)and cassava distillery residue (cassava stillage and its supernatant)were chosen as four kinds of fermentation substrates. Meanwhile Candida utilis and Geotrichum candidum were chosen as strains. Batch fermentation was applied to study the effect of substrates and strains on SCP production. The experimental results showed that yellow winery residue was suitable for SCP production. The maximum SCP yield was 90.22g/L with production rate of 3.91 and crude protein content of 53.3%, after 4 days of fungal fermentation via Candida utilis and Geotrichum candidum co-culture in 6% yellow wine lees-rice wastewater substrate. Furthermore, the synthesized SCP product contains essential amino acids, which could be widely used as animal feed in the future.