| 高效液相色谱法测定枸杞叶中精胺和亚精胺 |
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| 引用本文： | 马宝龙,屈旭斌. 高效液相色谱法测定枸杞叶中精胺和亚精胺[J]. 宁夏工程技术, 2011, 10(1): 48-50 |
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| 作者姓名： | 马宝龙 屈旭斌 |
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| 作者单位： | 1. 宁夏大学,化学化工学院,宁夏,银川,750021;神华宁夏煤业集团有限责任公司,宁夏,银川,750011
2. 宁夏大学,化学化工学院,宁夏,银川,750021 |
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| 摘 要： | 为了建立枸杞叶中精胺和亚精胺含量测定方法,对苯甲酰氯衍生精胺和亚精胺高效液相色谱法的分析条件进行了探讨,提出了精胺和亚精胺色谱测定的优化条件.结果表明,在苯甲酰氯衍生剂用量7μL,流动相V(甲醇)∶V(水)=70∶30,流速1.0 mL/min,检测波长230 nm和柱温30℃的条件下,测定方法有较好的准确度和灵敏度.精胺和亚精胺的回收率分别为105.0%,95.9%,方法检测限分别为0.167,1.060nmoL/mL,标准偏差分别为6.7%,5.3%.
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| 关 键 词： | 精胺 亚精胺 高效液相色谱法 衍生化 |
Determination of spermine and spermidine in wolfberry leaves by HPLC |
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| MA Baolong,QU Xubin. Determination of spermine and spermidine in wolfberry leaves by HPLC[J]. Ningxia Engineering Technology, 2011, 10(1): 48-50 |
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| Authors: | MA Baolong QU Xubin |
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| Affiliation: | 1.School of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China; 2.Shenhua Ningxia Coal Industry Group Co.,Ltd., Yinchuan 750011,China) |
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| Abstract: | In order to establish the determination method of spermine and spermidine in wolfberry leaves, analytical condition is approached and put forward optimal condition on HPLC for chloride derivatives of spermine and spermidine. Result is shown that the satisfactory analysis method with better accuracy and sensitivity is under the ratio of methanol to water in mobile phase 70：30, flow rate of 1.0 mL/min, volume of benzoyl chloride 7 uL and column temperature 30℃. The recoveries of spermine and spermidine are 105.0% and 95.9%, respectively, limit of detection are 0.167 nmol/mL and 1.060 nmol/mL, relative standard deviation are 6.7% and 5.3%. |
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| Keywords: | spermine spermidine HPLC derivatization |
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