| 苦豆子胶多糖组分气相色谱与薄层色谱分析 |
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| 引用本文： | 杨永利,郭守军,罗军德,马瑞君.苦豆子胶多糖组分气相色谱与薄层色谱分析[J].食品研究与开发,2006,27(11):159-162. |
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| 作者姓名： | 杨永利 郭守军 罗军德 马瑞君 |
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| 作者单位： | 1. 韩山师范学院生物系,广东,潮州,541041
2. 兰州大学生命科学学院,甘肃,兰州,730000 |
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| 摘 要： | 利用铜沉和醇沉法提取纯化多糖，以薄层色谱和气相色谱法进行定性和定量分析，建立苦豆子胶多糖组分分析方法。结果苦豆子胶多糖是由甘露糖和半乳糖组成，其百分舍量分别为39．9395％和17．8849％；相对标准偏差为3．5602％-5.0763％，回收率为98．63％-99．68％；二者配比为半：甘=1：2．23；总糖含量为57．8244％：结论：该方法便捷，可靠，可作为苦豆子胶多糖组分定性和定量的分析方法．
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| 关 键 词： | 苦豆子 苦豆子胶 多糖 薄层色谱 气相色谱 |
| 收稿时间： | 2006-07-14 |
| 修稿时间： | 2006年7月14日 |
ANALYSIS OF POLYSACCHARIDE COMPOSITION OF SOPHORA ALOPECUROIDES SEED BY GC AND TLC |
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| YANG Yong-li,GUO Shou-jun,LUO Jun-de,MA Rui-jun.ANALYSIS OF POLYSACCHARIDE COMPOSITION OF SOPHORA ALOPECUROIDES SEED BY GC AND TLC[J].Food Research and Developent,2006,27(11):159-162. |
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| Authors: | YANG Yong-li GUO Shou-jun LUO Jun-de MA Rui-jun |
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| Affiliation: | 1. Hanshan Normal College, Chaozhou 541041, Guangdong, China; 2. Lanzhou University, Lanzhou 730000, Gansu, China |
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| Abstract: | To establish an analysis method of saccharide components. The saccharide was extracted with hot water, and purified with Fehling reagent and 95 % ethanol; the saccharide components of sophora alopecuroides gum was analysized by TLC and GC. The polysaccharide of sophora alopecuroides gum was composed of D-galactose and D-mannose at ratio 1 : 2.23, and their percentage are 17.884 9 % and 39.939 5 %, respectively, with a relative standard deviation of 3.560 2 % ~ 5.076 3 %, and the recovery 98.63 %~99.68 % . The method is reliabale, and can be used for a qualitative and quantitative method of saccharide components of sophora alopecuroides gum. |
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| Keywords: | Sophora alopecuroides L sophora alopecuroides gum saccharide components TLC GC |
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