阿霉素耐药的乳腺癌MCF-7／Adm细胞系的建立及其耐药特性

建立肿瘤耐药细胞系是研究肿瘤细胞的重要手段之一，也是探讨肿瘤多药耐药机制及其逆转的基础。采用大剂量间歇诱导法诱导 MCF-7细胞，建立 MCF-7/Adm耐药模型；MTT 法检测耐药倍数；流式细胞术检测MCF-7细胞和MCF-7/Adm细胞中阿霉素的积累量；real-time PCR检测MCF-7/Adm细胞中 A BCG2、A BCC1、A B-CB1基因的表达。结果显示，阿霉素抑制 MCF-7和 MCF-7/Adm 细胞存活的 IC50值分别为0．535μg/mL 和173.275μg/mL ，耐药倍数为324；MCF-7细胞中的阿霉素积累量较MCF-7/Adm明显高；MCF-7/Adm细胞中mR-NA水平 A BCB1基因表达明显上调，A BCG2和 A BCC1基因表达下调。成功获得对阿霉素耐药的人乳腺癌耐药细胞株MCF-7/Adm ，为进一步研究乳腺癌多药耐药机制及其逆转提供有利工具，并且对乳腺癌化疗药物的筛选具有一定的意义。

Establishment of Adriamycin-Resistant Breast Cancer Mcf-7/Adm Cell Line and the Drug Resistance Feature

Establishment of tumor drug-resistant cell lines is an important method to study tumor cells and is also the foundation of exploring tumor multidrug-resistant mechanism and the reverse .This paper a-dopted high-dose intermittent deduction method to induce MCF-7 cells and establish MCF-7/Adm drug-re-sistance model .MTT method is used to detect the drug-resistance times .Flow cytometry is used to detect the accumulation of adriamycin in MCF-7 cell and MCF-7/Adm cell .Real-time PCR was adopted to detect the genetic expression of ABCB1 ,ABCC1 and ABCG2 in MCF-7/Adm cell .The results show IC50 values of MCF-7 and MCF-7/Adm cells restrained by adriamycin are 0 .535 and 173 .275μg/mL respectively ,with drug-resistance times of 324 ;adriamycin accumulation in MCF-7 cells was significantly higher than that in MCF-7/Adm cells ;genetic expression of A BCB1 in MCF-7/Adm cells at mRNA level up-regulated obvi-ously ;genetic expression of ABCC1 and ABCG2 down-regulated .This paper successfully gains adriamy-cin-resistant breast cancer drug-resistance cell strain MCF-7/Adm which provides a helpful tool for stud-ying breast cancer multidrug resistance mechanism and the reverse .It is also significant to choose effective anti-caner drugs .