Modified technique for efficient detection of microsporidia |
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Authors: | E Kokoskin TW Gyorkos A Camus L Cedilotte T Purtill B Ward |
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Affiliation: | McGill University Centre for Tropical Diseases, Montreal General Hospital, Canada. |
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Abstract: | Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min. |
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