| 抗坏血酸及L-半胱氨酸的流动注射动力学同时测定法 |
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| 引用本文： | 曾飒,谭辉玲.抗坏血酸及L-半胱氨酸的流动注射动力学同时测定法[J].食品科学,2000,21(2):60-62. |
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| 作者姓名： | 曾飒 谭辉玲 |
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| 作者单位： | 1. 中山医科大学化学教研室,广州510089
2. 重庆大学化工学院,630044 |
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| 摘 要： | ｐＨ２￣９时，抗坏血酸与Ｌ－半胱氨酸均可与Ｆｅ＾３＋－邻菲咯啉混合液反应，生成Ｆｅ＾２＋－邻菲咯啉有色配合物，但二者反应速度相差极大，根据这个原理，本研究采用流动注射分析停流技术，建立了抗坏血酸与Ｌ－半胱氨酸的动力学分光光度同时测定法。
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| 关 键 词： | 同时测定 抗坏血酸 L－半胱氨酸 FIA停流技术 |
Simultaneous Determination of VC and L-cys in Fruit Juices by Stopped-Flow Kinetic Spectrophotometry |
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| Abstract.Simultaneous Determination of VC and L-cys in Fruit Juices by Stopped-Flow Kinetic Spectrophotometry[J].Food Science,2000,21(2):60-62. |
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| Authors: | Abstract |
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| Affiliation: | Zeng Sa et al |
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| Abstract: | Astopped-flow kinetic spectrophotometric method is proposed for the simultaneous determination of ascorbic acid (Vc ) and L-cysteine(L-cys)in fruit juices.The calibration graphs are linear for 0-24.0-280mg/L and the detection limits are lmg/ L and 16mg/L for Vc and L-cys.respectively.The injectd sample volume is 157uL and the sampling rate is 30 samples/h.This method is simple rapid. sensitive and accurate with high selectivity and has been applied for determination of Vc and L-cys in strawberry juice.The marked recovery rates are up to 95.2%and 92.8%for Vc and L-cys respectivily. |
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| Keywords: | Stopped Folw Analysis Simultaneous Determination Spectrophotometric method Ascorbic acid L-cysteine |
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