In vitro assessment of grapevine resistance to two populations of phylloxera from Australian vineyards |
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| Authors: | ALISON V. KELLOW GARRICK McDONALD ANGELA M. CORRIE ROBYNVAN HEESWIJCK |
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| Affiliation: | University of Adelaide (Waite Campus), Department of Horticulture Viticulture and Oenology, PMB 1, Glen Osmond SA 5064, Australia;Cooperative Research Centre for Viticulture, PO Box 154, Glen Osmond SA 5064, Australia;Agriculture Victoria, Rutherglen, RMB 1145, Rutherglen Victoria 3685, Australia |
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| Abstract: | A perlite-based medium is described for in vitro cocultivation of phylloxera with micropropagated vines. Using this system, as well as cocultivations on excised primary roots, six vine types were screened for resistance to one or two populations of phylloxera (SRU-1 or VWL-1) sourced from different Australian vineyards. When inoculated with VWL-1 phylloxera, V. vinifera cv. Shiraz was rated as susceptible, Ramsey as resistant, Schwarzmann, V. riparia and Börner as highly resistant and V. rotundifolia as immune. When inoculated with SRU-1 phylloxera, both V. vinifera and Schwarzmann were rated as susceptible. The potential use of these in vitro methods for rootstock resistance screening and determination of phylloxera biotypes is discussed. Phylloxera behaviour and root responses observed in vitro are also described. |
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| Keywords: | grapevine phylloxera biotype phylloxera resistance rootstock nodosity tissue culture in vitro assessment |
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