| 枯草芽孢杆菌产果胶裂解酶培养基优化研究 |
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| 引用本文： | 杨秀松,张新平. 枯草芽孢杆菌产果胶裂解酶培养基优化研究[J]. 上海第二工业大学学报, 2012, 0(2): 105-111 |
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| 作者姓名： | 杨秀松 张新平 |
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| 作者单位： | 1. 国家食品药品监督管理局高级研修学院,北京100073
2. 上海交通大学农业与生物学院,上海201101 |
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| 摘 要： | 通过Plackett-Burman试验设计,从葡萄糖、果糖、豆粕粉、氯化铵、乙酸铵、硫酸铵、蛋白胨、酵母提取物、K2PHO4、KH2PO4、CaCl2等11个相关因子中筛选出对枯草芽孢杆菌产果胶裂解具有显著影响的因子——果糖、豆粕和酵母提取物,选取因子豆粕和酵母提取物进行响应面设计分析,研究结果表明当豆粕和酵母提取物含量分别为100 g/L,25 g/L时,果胶裂解酶活性最大为22.69 U/mL。
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| 关 键 词： | 枯草芽孢杆菌 果胶裂解酶 中心组合设计 |
Optimization of Culture Components for Pectinase Production by Bacillus Subtilis |
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| YANG Xiu-song,ZHANG Xin-ping. Optimization of Culture Components for Pectinase Production by Bacillus Subtilis[J]. Journal of Shanghai Second Polytechnic University, 2012, 0(2): 105-111 |
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| Authors: | YANG Xiu-song ZHANG Xin-ping |
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| Affiliation: | 1. State Food and Drug Administration Institute of Executive Development, Beijing 100073, P. R. China; 2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, P. R. China ) |
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| Abstract: | A Plackett-Burman design was used to screen the significant factors from glucose, sucrose, soybean meal, NH4Cl, （NH4）2CHCOOH, （NH4） SO4, peptone, yeast extracts, K2PHO4, KH2PO4 and CaCl2 to affect pectinase production. Furthermore, central composite design of experiments was carried out to optimize the optimal levels of two important factor, namely soybean meal and yeast extracts. The optimum concentration levels for obtaining maximum 22.69 U/mL pectinase activity were： 100 g/L soybean meal and 25 g/L yeast extracts. |
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| Keywords: | Bacillus Subtilis pectinase central composite design |
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