| 高效液相色谱法同时测定口腔护理用品中3种抗菌药物 |
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| 引用本文： | 车文军,蒋星仪,钱凯,孙姗姗,吴金兰.高效液相色谱法同时测定口腔护理用品中3种抗菌药物[J].化工时刊,2014(10):19-21,54. |
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| 作者姓名： | 车文军 蒋星仪 钱凯 孙姗姗 吴金兰 |
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| 作者单位： | 1. 江苏省产品质量监督检验研究院,江苏 南京,210007
2. 南京农业大学食品科技学院,江苏 南京,210095 |
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| 摘 要： | 建立高效液相色谱（ HPLC）同时测定口腔护理用品中3种抗菌药物含量的分析方法。试样经甲醇水溶液超声提取后,以甲醇-磷酸盐缓冲溶液为流动相,经C18柱分离后进行HPLC检测。3种药物在2.0mg/L-100mg/L范围内呈良好的线性关系,线性相关系数均大于0.999。20、40、100 mg/kg 3个浓度加标水平的平均回收率为97.2%-103.7%,相对标准偏差（RSD,n=6）为1.28%-2.36%,检出限为2.5 mg/kg-5.0mg/kg。该方法灵敏度高、重现性好、定量准确。
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| 关 键 词： | 高效液相色谱 口腔护理用品 抗菌药物 测定 |
Simultaneous Determination of Three Antibacterial Drugs in Oral Care Products by HPLC |
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| Che Wenjun,Jiang Xingyi,Qian Kai,Sun Shanshan,Wu Jinlan.Simultaneous Determination of Three Antibacterial Drugs in Oral Care Products by HPLC[J].Chemical Industry Times,2014(10):19-21,54. |
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| Authors: | Che Wenjun Jiang Xingyi Qian Kai Sun Shanshan Wu Jinlan |
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| Affiliation: | Che Wenjun;Jiang Xingyi;Qian Kai;Sun Shanshan;Wu Jinlan;Jiangsu Product Quality Testing and Inspection Institute;College of Food Science and Technology,Nanjing Agricultural University; |
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| Abstract: | A high performance liquid chromatography （ HPLC） method was developed for simultaneous determi-nation of three antibacterial drugs in oral care products. The samples were extracted with methanol-water solution under ultrasonication. The separation of three antibacterial drugs was carried out on a C18 column using methanol-phosphate buffer as mobile phase. For the three drugs, the calibration curves were linear between 2. 0 to 100 mg/L, and the correlation coefficients were more than 0 . 999 . The mean recoveries at spiked levels of 20、40、100 mg/kg were in the range of 97. 2% -103. 7% ,RSDs（n=6）of 1. 28% -2. 36%. The limit of detection were in the range of 2. 5 mg/kg-5. 0 mg/kg. The method was sensitive, reproducible and accurate. |
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| Keywords: | high performance liquid chromatography ( HPLC) oral care products antibacterial drugs deter-mination |
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