Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood |
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| Authors: | Ilgım Göktürk Ali Derazshamshir Fatma Yılmaz Adil Denizli |
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| Affiliation: | 1. Division of Nanotechnology and Nanomedicine, Hacettepe University, Beytepe, Ankara, Turkey;2. Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkeydenizli@hacettepe.edu.tr;4. Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkey;5. Department of Chemistry Technology, Vocational School of Gerede, Bolu, Turkey |
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| Abstract: | We have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated. |
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| Keywords: | Cryogel haemoglobin IMAC PEI-Cu2+ PVA |
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