Molecular profiling of Jatropha curcas L. collected from different geographical locations of India |
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| Authors: | K.C. Verma U.S. Singh S.K. Verma A.K. Gaur |
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| Affiliation: | 1. Lovely Professional University, Ludhiana – Jalandhar G.T. Road, Phagwara 144402, Punjab, Indiakcbiochem@rediffmail.comkcbiochem@gmail.com;4. IRRI, NASC Complex, SAAS Building, New Delhi 110012, India;5. Department of Plant Breeding, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, Uttarakhand, India;6. Department of Biochemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India |
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| Abstract: | The present study surveys the morphological, biochemical and molecular diversity in 30 accessions of Jatropha collected from different states of India by using random amplified polymorphic DNA (RAPD), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and isozyme analysis. The genotyping data were used to understand the relationships among accessions and to identify genetic diversity as a means for genetic improvement of Jatropha. Out of 37 decamer primers used, 18 yielded polymorphic banding pattern. In total, 126 different DNA bands were reproducibly obtained, out of which 81 were polymorphic. SDS-PAGE of seed, leaf and callus resolved into 18, 12 and 7 bands, while no biotype-specific band was found in leaf and callus protein profile. Cluster analysis of both RAPD and SDS-PAGE data produced two major clusters. |
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| Keywords: | biotype Jatropha curcas RAPD SDS-PAGE biochemical parameters |
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