Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9
Abstract:
Defatted canola meal was extracted with 95% (v/v) ethanol at 80 °C. The extract was fractionated on a Sephadex LH-20 column using methanol as eluate. Seven major fractions were isolated according to UV absorption, content of phenolics and sugars. Antioxidant activity of these fractions was evaluated in a β-carotenelinoleate model system. Fraction IV showed the best antioxidant effect by exhibiting the highest preventive activity against the bleaching of β-carotene. Further separation of this fraction on TLC indicated that it contains several compounds including phenolic acids and trihydroxy phenolic compounds such as flavones and flavonols.