Determination of the biodegradable fraction of dissolved organic matter in waters |
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| Authors: | Pierre Servais, Gilles Billen,Marie-Claude Hascoë t |
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| Affiliation: | 1. Groupe de Microbiologie des Milieux Aquatiques, Université Libre de Bruxelles, 50, avenue Franklin D. Roosevelt, B-1050 Bruxelles, Belgium;2. Compagnie Générale des Eaux 52, rue d''Anjou, F-75384 Paris Cedex 08, France;1. Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China;2. Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China;3. Collaborative Innovation Center for Advanced Nuclear Energy Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China;4. Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Institute, Shenzhen 518055, PR China;1. CETaqua, Water Technology Center, Carretera d''Esplugues 75, 08940 Cornellà de Llobregat, Barcelona, Spain;2. Chemical Engineering Department, Technical University of Catalonia (UPC), Av. Diagonal 647, 08028 Barcelona, Spain;3. SGAB, Societat General d’1Aigües de Barcelona, Av. Diagonal 211, 08018 Barcelona, Spain |
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| Abstract: | Two bioassay procedures are proposed for determining biodegradable dissolved organic carbon (BDOC) in waters. Both involves sterile filtration of the sample, reinoculation with a natural assemblage of bacteria from the same origin as the sample, and incubation for at least 10 days in the dark at 20°C. In the first procedure, dissolved organic carbon (DOC) is followed, until a plateau is reached and the difference between initial and final DOC is taken as a measure of BDOC. In the second procedure, bacterial biomass and mortality rate are followed and the integrated flux of mortality during the incubation period is calculated and divided by the growth yield to give an estimate of BDOC. Both procedures provide closely concordant results.An example of application to the study of ozonation and biological activated carbon filtration in drinking water treatment is presented. |
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| Keywords: | biodegradation organic matter bacteria ozone activated carbon bioassay drinking water |
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