Functional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas reinhardtii |
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Authors: | Renaud LeclerDamien Godaux Hélène VigeolasSerge Hiligsmann Philippe ThonartFabrice Franck Pierre CardolClaire Remacle |
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Affiliation: | a Laboratory of Genetics of Microorganisms, University of Liege, Bvd du Rectorat 27, B-4000 Liège, Belgium b Walloon Center for Industrial Biology (CWBI), University of Liege, Bvd du Rectorat 29, B-4000 Liège, Belgium c Laboratory of Plant Biochemistry and Photobiology, University of Liege, Bvd du Rectorat 27, B-4000 Liège, Belgium |
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Abstract: | In this paper, mitochondrial mutants of Chlamydomonas reinhardtii defective for respiratory complex I (NADH:ubiquinone oxidoreductase), complex III (ubiquinol cytochrome c oxidoreductase) and both complexes I and III were analyzed for H2 photoproduction. Several parameters were followed during the S-deficiency stage and the anaerobic stage leading to H2 photoproduction. At the early aerobic S-deficiency stage, starch and neutral lipids accumulated in all strains but their amount was significantly decreased in mutants compared to wild type. During the H2 photoproduction process, whereas starch content strongly decreased in all strains, neutral lipid amount remained nearly unchanged, suggesting that starch degraded by glycolysis is the preferential substrate for energy production during anaerobiosis. The mutants displayed a decrease in H2 photoproduction correlating to the number of active mitochondrial proton-pumping sites lost in the strains. Our results thus highlight the critical role of oxidative phosphorylation during the first (aerobic) stage of S-starvation when carbon resources are accumulated. |
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Keywords: | Chlamydomonas Respiratory-deficient mutant Photohydrogen Oxidative phosphorylation Starch Neutral lipids |
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