一点红黄酮类化合物分离及其抗菌性能研究

本实验采用70%的乙醇水溶液回流提取一点红中的黄酮类化合物,然后通过石油醚萃取除掉其中色素等杂质。提取物进一步通过聚酰胺薄板进行薄层色谱分析[展层剂为正丁醇:水:醋(10:5:1)],且通过聚酰胺柱进行分离纯化(洗脱液为70%乙醇水溶液)。薄层色谱分析结果(以芦丁标样为对照)以及相关的黄酮化学检识实验结果表明,一点红黄酮类化合物具有与芦丁成分相似的黄酮类化合物和较高的纯度,对金黄色葡萄球菌具有较强的抑菌作用,对大肠杆菌及枯草杆菌的抑菌效果次之。

Study on Separations of Emilia sonchifolia Flavonoids and Their Antibacterial Activities

The flavonoids of Emilia sonchifolia were firstly extracted by 70% alcohol-water and then petroleum ether were used to eliminate the pigment or other ingredients inside.The Emilia sonchifolia flavonoid extracts were purified by the polyamide thin layer chromatography(TLC)(the developing solvent is n-butyl alcohol:H2O:acetic acid=10:5:1)or by polyamide column(70% aqueous ethanol as the eluent).The results of TLC(rutin as control)and flavonoid identification experiments showed that the the Emilia sonchifolia flavonoid has a similar components with rutin flavonoids and higher purity.The Emilia sonchifolia flavonoid showed stronger antibacterial activity against Staphylococcus aureus and weaker antibacterial activitiy against Escherichia coli andB acillus subtilis.