燃料油的生物脱硫

从大港油田的污泥中分离出一株能以二苯并噻吩(DBT)为唯一硫源而生长的菌株(Rhodococcussp.EBT-2)。HPLC分析结果表明,EBT-2可专一性降解DBT,生成2-羟基联苯(2-HBP)。以DBT为唯一硫源考察了培养基的初始pH、温度、碳源和氮源等因素对EBT-2生长和脱硫的影响。结果表明,EBT-2的最适初始pH为7.5,最适温度为30℃,最佳碳、氮源分别是葡萄糖和氯化铵。在最适条件下,EBT-2能够在48 h内将DBT从0.25 mmol/L降解到0.03 mmol/L,同时生成0.19 mmol/L 2-HBP。

Biodesulfurization of Fuel Oils

An organism,identified as Rhodococcus sp.EBT-2,was isolated from the slurry in Dagang Oil Field of Tianjin,China.Analysis of HPLC revealed that EBT-2 could desulfurize DBT to 2-hydroxybiphenyl(2-HBP) selectively.Furthermore,the effects of temperature,initial pH,carbon source and nitrate source on the growth and capacity of removal DBT were investigated.Results showed that the suitable inital pH is 7.5,temperature is 30 ℃,the best carbon source and nitrate source are glucose and ammonium chloride.Under optimum operating conditions,DBT of 0.25 mmol/L was degraded to 0.03 mmol/L by growing cells of EBT-2 within 48 h,simultaneously producing 2-HBP of 0.19 mmol/L.