| 可见分光光度法快速检测碳氧血红蛋白饱和度 |
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| 引用本文： | 杨娅,屠一锋. 可见分光光度法快速检测碳氧血红蛋白饱和度[J]. 现代科学仪器, 2008, 0(6) |
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| 作者姓名： | 杨娅 屠一锋 |
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| 作者单位： | 1. 苏州大学基础医学与生物科学学院法医教研室,苏州,215123;苏州大学化学化工学院分析化学研究所,苏州,215123
2. 苏州大学化学化工学院分析化学研究所,苏州,215123 |
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| 摘 要： | 建立了用紫外-可见分光光度计快速检测血液中碳氧血红蛋白饱和度的方法.检血用0.1%Na2CO3稀释后加入连二亚硫酸钠,用紫外-可见分光光度计测定500nm-600nm吸收光谱图和419nm及425.5nm处的吸光度值,对碳氧血红蛋白进行定性和定量分析.该法检测血液中碳氧血红蛋白饱和度具有快速、准确、操作简便等优点,可用于临床CO中毒的快速诊断及CO中毒死亡的法医学鉴定.
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| 关 键 词： | 紫外-可见分光光度法 一氧化碳中毒 碳氧血红蛋白 快速检测 |
Rapid Detection of Carboxyhemoglobin by Spectrophotometry |
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| Yang Ya,Tu Yifeng. Rapid Detection of Carboxyhemoglobin by Spectrophotometry[J]. Modern Scientific Instruments, 2008, 0(6) |
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| Authors: | Yang Ya Tu Yifeng |
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| Affiliation: | Yang Ya~(1,2) Tu Yifeng~2 (1 Institute of Forensic Medicine,College of Basal Medicine , Bioscience,Suzhou,215123,China) (2 Institute of Analytical Chemistry,College of Chemistry , Chemical Engineering Suzhou University,China) |
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| Abstract: | This paper reported a new developed method for identification and determination of earboxyhemo- globin in carbon monoxide poisoned blood by spectrophotometry.By addition of suitable amount of sodium hydro- sulfite into blood samples which were diluted by 0.1% Na_2CO_3,the absorption curve from 400nm to 600nm and absorbance at 419 nm and 425.5nm of the blood samples were detected for qualitative and quantitative determina- tion of carboxyhemoglobin.The method is more simple,accurate,rapid and practical.It ca... |
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| Keywords: | Spectrophotometry Carboxyhemoglobin Carbon monoxide poisoning Rapid detection |
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