Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3 |
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| Authors: | Changqing Zhang Guangping Li Jin Wang Shinong Zhu Hailing Li |
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| Affiliation: | 1.College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China; E-Mails: (S.Z.); (H.L.);2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; E-Mail: ;3.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China; E-Mail: |
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| Abstract: | The production of small RNAs (sRNAs) from phased positions set by microRNA-directed cleavage of trans-acting-siRNA-producing locus (TAS) transcript has been characterized extensively; however, the production of sRNAs from non-phased positions remains unknown. We report three cis-cleavages that occurred in TAS3 transcripts in Vitis vinifera, by combining high-throughput sRNA deep sequencing information with evolutional conservation and genome-wide RNA degradome analysis. The three cis-cleavages can be deciphered to generate an orderly cleavage cascade, and can also produce distinct phasing patterns. Each of the patterns, either upstream or downstream of the cis-cleaved position, had a set of sRNAs arranged in 21-nucleotide increments. Part of the cascading cis-cleavages was also conserved in Arabidopsis thaliana. Our results will enhance the understanding of the production of sRNAs from non-phased positions that are not set by microRNA-directed cleavage. |
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| Keywords: | trans-acting siRNA cis-cleavage ta-siRNA-producing locus miRNA |
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