An automated enzymic micromethod for the measurement of fat in human milk |
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| Authors: | A Lucas G J Hudson P Simpson T J Cole B A Baker |
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| Affiliation: | Medical Research Council, Dunn Nutrition Unit, Cambridge, UK. |
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| Abstract: | Most (98%) of the fat in human milk is present as triglycerides. This paper describes the use of a clarification procedure that enables the level of human milk fat to be determined by measurement of glycerol released by enzymic hydrolysis of triglycerides. The method requires only 10-50 microliters milk, thus presenting a possible technique for work with small mammals, and is suitable for use with autoanalysers, permitting rapid sample throughput. |
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