| 婴儿配方奶粉中叶黄素测定 |
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| 引用本文： | 陶大利.婴儿配方奶粉中叶黄素测定[J].生命科学仪器,2011,9(5):36-38. |
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| 作者姓名： | 陶大利 |
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| 作者单位： | 黑龙江省农垦乳品检测中心,黑龙江省哈尔滨,150078 |
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| 摘 要： | 建立高效液相色谱法测定婴儿配方奶粉中叶黄素的检测方法。采用色谱柱为Carotenoid C30色谱柱（250.0mm×4.6mm,5μm）,流动相为甲醇和甲基叔丁基醚,梯度洗脱,紫外检测器,检测波长445nm。结果表明,叶黄素浓度在0.05～2.0μg/mL之间呈线性关系,线性方程为：y=379874.03X＋14289.56,相关系数R2=0.9990;奶粉的含量为50～500μg/100g时,回收率在92.8～103.5%之间,RSD%在1.2～3.5%之间;检出限为5μg/100g。该方法操作快捷简便,结果准确。
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| 关 键 词： | 高效液相色谱 奶粉 叶黄素 萃取 |
Determination of lutein in infant formula milk powder |
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| Tao DA-lI.Determination of lutein in infant formula milk powder[J].Life Science Instruments,2011,9(5):36-38. |
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| Authors: | Tao DA-lI |
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| Affiliation: | Tao DA-lI(1.Heilongjiang Agriculture Dairy Testing Center,Harbin 150078,China) |
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| Abstract: | A method for determination of lutein in infant formula milk powder by HPLC is reported.HPLC on a Carotenoid C30 column（250.0 mm×4.6mm,5μm） with methanol-MTBE gradient elution,UV-vis detector,wavelength at 445nm for determenation of lutein.Linear ranges of lutein was 0.05～2.0μg/mL,the linear equation was：y=379874.03X＋14289.56,relevant index R2=0.9990;the content of milk powder were 50～500μg/100g,the recovery rate were 92.8～103.5%,and relative standard deviation was 1.2～3.5,the detectabke value is 5μg/100g.This method is fast,simple and accurate. |
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| Keywords: | HPLC milk powder l utein extraction |
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