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Mass transfer of anti-cancer drug delivery to brain tumors by a multiple emulsion-based implant |
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| Authors: | Ewa Dluska Agnieszka Markowska-Radomska Agata Metera Leszek Rudniak Konrad Kosicki |
| Affiliation: | 1. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland;2. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland Contribution: Conceptualization (supporting), Data curation (equal), Formal analysis (equal), Funding acquisition (supporting), Investigation (equal), Methodology (equal), Resources (supporting), Supervision (supporting), Validation (supporting), Visualization (equal), Writing - original draft (supporting), Writing - review & editing (supporting);3. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland Contribution: Conceptualization (supporting), Data curation (supporting), Formal analysis (supporting), Funding acquisition (supporting), Investigation (equal), Methodology (supporting), Resources (supporting), Supervision (supporting), Validation (supporting), Visualization (equal), Writing - original draft (supporting), Writing - review & editing (supporting);4. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland Contribution: Conceptualization (supporting), Data curation (supporting), Formal analysis (supporting), Funding acquisition (supporting), Investigation (supporting), Methodology (supporting), Resources (supporting), Supervision (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting), Writing - review & editing (supporting);5. Faculty of Biology, Institute of Genetics & Biotechnology, University of Warsaw, Warsaw, Poland Contribution: Conceptualization (supporting), Data curation (supporting), Formal analysis (supporting), Funding acquisition (supporting), Investigation (supporting), Methodology (supporting), Resources (supporting), Supervision (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting), Writing - review & editing (supporting) |
| Abstract: | The advanced use of a pH-responsive biomaterial-based injectable liquid implant for effective chemotherapeutic delivery in glioblastoma multiforme (GBM) brain tumor treatment is presented. As an implant, we proposed a water-in-oil-in-water multiple emulsion with encapsulated doxorubicin. The effectiveness of the proposed therapy was evaluated by comparing the cancer cell viability achieved in classical therapy (chemotherapeutic solution). The experimental study included doxorubicin release rates and consumption for two emulsions differing in drop sizes and structures in the presence of GBM-cells (LN229, U87 MG), and a cell viability. The results showed that the multiple emulsion implant was significantly more effective than classical therapy when considering the reduction in cancer cell viability: 85% for the emulsion-implant, and only 43% for the classical therapy. A diffusion–reaction model was adapted to predict doxorubicin release kinetics and elimination by glioblastoma cells. CFD (computational fluid dynamics) simulations confirmed that the drug release kinetics depends on multiple emulsion structures and drop sizes. |
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