黑牛肝菌多糖对小鼠酒精性肝损伤的改善作用

目的:旨在研究黑牛肝菌多糖（refined polysaccharide from Boletus aereus,RPBA）对小鼠酒精性肝损伤的保护作用。方法:将60只小鼠适应性喂养3 d后,随机分为空白对照组、阳性对照组（联苯双酯滴丸,150 mg/kg·bw）、模型组、RPBA各剂量组（100、200、400 mg/kg·bw）,连续灌胃30 d,第31 d禁食不禁水12 h,除空白组外的其余各组灌胃50%乙醇溶液（12 m L/kg·bw）,建立动物急性肝损伤模型。结果:小鼠酒精灌胃后,模型组谷丙转氨酶（ALT）、谷草转氨酶（AST）、甘油三酯（TG）水平及丙二醛（MDA）含量与空白对照组相比显著（p<0.05）升高,超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活力、还原型谷胱甘肽（GSH）含量显著（p<0.05）低于空白对照组,RPBA各剂量组ALT、AST、TG水平及MDA含量与模型组相比明显降低,而以上各抗氧化酶活性明显提高。结论:RPBA能明显降低小鼠血清中AST、ALT及TG水平,提高血清中抗氧化酶活性,减少脂质过氧化,对小鼠酒精性肝损伤具有明显的保护作用。 

Improvement effect of polysaccharides from Boletus aereus on alcoholic liver injury in mice serum

Objective : The improvement effect of refined polysaccharide from Boletus aereus( RPBA) on acutealcoholic hepatic injury in mice was investigated. Methods:The 60 mice were fed adaptively for 3 days,then were randomly divided into six groups,including blank control group,alcoholic model group,positive control group(administered with 150 mg/kg·bw bifendate by gavage),and RPBA(100,200,400 mg/kg·bw)group. After housing for 30 days,the mice were kept fasting but retained water for 12 hours,then 50% alcohol(12 m L/kg·bw) were administered orally into animals of all groups except blank control group to induce hepatotoxicity. Results:The results showed that the levels of ALT,AST,TG and MDA of mice in model group were incereased obviously(p <0.05),while the levels of SOD,CAT,GSH-Px,and GSH were lower(p <0.05)compared with the control group. After BAP administration,the levels of AST,ALT and MDA were decreased while the levels of SOD,CAT,GSH-Px,and GSH were increased. Conclusion:RPBA could significantly reduce the levels of AST,ALT and TG in serum of mice,increase the activities of antioxidant enzymes in serum,and reduce lipid peroxidation.RPBA could protect liver from injury induced by alcohol in mice.