菜籽多糖对S180小鼠肉瘤及其免疫能力的影响

研究菜籽多糖的抑瘤作用及其机理。通过接种S180细胞复制荷S180瘤小鼠模型,分别腹膜注射生理盐水、环磷酰胺、菜籽多糖,测定各组小鼠肿瘤重量与脏器指数、免疫能力、抗氧化能力、乳酸脱氢酶活力等指标。结果表明:50～200mg/kg·d的RSPS抑瘤率在20．66%～34．710%之间;RSPS能显著提高荷瘤小鼠脾脏指数、腹腔巨噬细胞吞噬率与吞噬指数、迟发性超敏反应、小鼠血清溶血素含量与脾细胞抗体形成等指标,显著抑制荷S?小鼠血清LDH的活性,提高红细胞CAT活性,降低血清中MDA含量。以上结果表明RSPS在小鼠体内具有抑制S180肉瘤的作用,能增进荷瘤小鼠免疫力、清除自由基与抑制LDH活性可能是其抑制肿瘤的机理。

Effects of Rapeseed Polysaccharides(RSPS) on S180 Tumor Growth Inhibition and Immune Capability Enhancement in vivo

Effects and mechanism of RSPS on S180tumor growth inhibition were studied in this paper.The tumor-bearing mice were gained by being implanted S180cells inoculated in right front axillas.The S180-bearing mice were injected with physiological saline,cyclophosphamide and RSPS into its intra-peritoneal.The indexes were detected for the weight of rumor and immune organ, immune function and antioxidation capability.Results showed that RSPS could inhibit tumor growth in vivo and the inhibiting rate was between 20.66% and 34.71% at 50～200mg/kg·d of RSPS.RSPS could also enhance immune capability,thymus index,spleen index,macrophage phagocytic rate and phagocytic index,delayed-type hypersensitivity,splenic antibody formation and serum hemolysin content,while inhibit mice lactate dehydrogenase activity,improve the mice catalase activity and decrease the MDA content in mice serum.The tumor inhibiting mechanism of RSPS can promote immunocompetence and antioxidative activities and inhibition of lactate dehydrogenase.