| 荧光定量PCR法检测人乳腺珠蛋白与乳腺癌微小转移诊断的研究 |
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| 作者单位： | 山东省临沂市第四人民医院 |
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| 摘 要： | 目的克隆人乳腺珠蛋白(hMaM)基因,建立荧光定量PCR方法,探讨外周血hMaM mRNA的表达作为乳腺癌血行微小转移标志物的可能性。方法设计特异引物,用RT-PCR方法从乳腺癌组织扩增获得目的片段,利用T/A克隆将PCR产物插入pMD-18T载体,优化扩增条件,以pMD/M质粒制备标准曲线,建立FQ-PCR方法,对乳腺癌不同分期的患者(n=28),乳腺良性疾病患者(n=15),其他肿瘤患者(n=33)的外周血进行检测。结果成功获得hMaM基因并完成克隆,阳性克隆测序结果与预期序列一致;建立了FQ-PCR检测hMaM mRNA方法;28例乳腺癌病人中9例(32.1%)阳性(Ⅰ期4例,Ⅱ期3例,Ⅲ期1例,Ⅳ期1例),hMaM mRNA的表达与乳腺癌病理分期无关(χ~2=0.5936;P>0.05);乳腺良性疾病组,其他肿瘤病人组及对照组外周血中均未检出hMaM mRNA。结论hMaM mRNA是乳腺癌特异性标志物,其外周血的表达可作为乳腺癌血行微小转移的指标。
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| 关 键 词： | 荧光定量PCR 乳腺珠蛋白 乳腺癌 微小转移 |
The Study of FQ-PCR for Determining Mammaglobin mRNA and Implication for Monitoring Micrometastasis of Patients with Breast Cancer |
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| GE Mingguang The Fourth People's Hospital of Lin'yi,Shan dong. The Study of FQ-PCR for Determining Mammaglobin mRNA and Implication for Monitoring Micrometastasis of Patients with Breast Cancer[J]. Friend of Chemical Industry, 2008, 0(28) |
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| Authors: | GE Mingguang The Fourth People's Hospital of Lin'yi Shan dong |
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| Affiliation: | GE Mingguang The Fourth People's Hospital of Lin'yi,Shan dong 276005 |
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| Abstract: | |
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| Keywords: | Fluorescence quantitative PCR Mammaglobin Breast cancer Micrometastasis |
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