| SAS9.0软件在均匀设计优化Streptomyces mediolani降解纤维素产乙醇发酵条件中的应用 |
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| 引用本文： | 解玉红,闵笛,刘双飞,赵亚男,冯炘. SAS9.0软件在均匀设计优化Streptomyces mediolani降解纤维素产乙醇发酵条件中的应用[J]. 酿酒, 2009, 36(2) |
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| 作者姓名： | 解玉红 闵笛 刘双飞 赵亚男 冯炘 |
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| 作者单位： | 天津理工大学环境科学与安全工程学院,天津,300384 |
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| 基金项目： | 天津市科委自然科学基金，天津市高校科技发展计划项目 |
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| 摘 要： | 选用梅久兰链霉菌(Streptomyces mediolani)K7-2作为试验菌株,采用均匀设计方法设计实验,利用分光光度法测定乙醇含量,用SAS9.0软件进行回归体积分数分析。经五步非线性逐步回归,得到了回归方程,决定系数为97.91%。通过极值计算,得到乙醇的最优产量为体积分数0.4430‰。此时,产生乙醇的发酵条件为温度为27℃,pH为8.5,接种量为10%。
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| 关 键 词： | 乙醇 发酵条件 均匀设计 SAS9.0软件 |
The Application of SAS9.0 in Fermentation Optimization of Streptomyces mediolani Degrading Cellulose into Ethanol by Uniform Design |
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| XIE Yu-hong,MIN Di,LIU Shuang-fei,ZHAO Ya-nan,FENG Xin. The Application of SAS9.0 in Fermentation Optimization of Streptomyces mediolani Degrading Cellulose into Ethanol by Uniform Design[J]. Liquor Making, 2009, 36(2) |
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| Authors: | XIE Yu-hong MIN Di LIU Shuang-fei ZHAO Ya-nan FENG Xin |
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| Affiliation: | the environmental and safety school of Tianjin Technology Universty;Tianjin;300384 |
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| Abstract: | In this paper,the Streptomyces mediolani K7- 2 was choiced for cultivated.The uniform design was used in the experiment, and the Spectrophotometry was used for measuring Ethanol content. Finally, thesoftware SAS9.0 was used for regression analysis. The R- Square of nonlinear regression is 97.91%. With calculating, the perfectethanol production was gotten, and it was 0.4430‰vol. At this time, the temperature of fermentation was 27℃, and the pH was8.5, and the inoculation was 10%. |
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| Keywords: | Ethanol Fermentation Uniform Design SAS9.0 |
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