Overexpression of a sugarcane<i> ScCaM</i> gene negatively regulates salinity and drought stress responses in transgenic <i>Arabidopsis thaliana</i> |
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| Authors: | JINXIAN LIU JINGFANG FENG CHANG ZHANG YONGJUAN REN WEIHUA SU GUANGHENG WU XIANYU FU NING HUANG YOUXIONG QUE HUI LING JUN LUO |
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| Affiliation: | 1.College of Tea and Food Science; Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, Wuyi University, Nanping, 354300, China
2 Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture; Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops,
Ministry of Education, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
3 College of Agriculture, Yulin Normal University, Yulin, 537000, China |
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| Abstract: | Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study,
the effects of a sugarcane ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in
transgenic Arabidopsis thaliana and Escherichia coli cells were evaluated. The results demonstrated a significant
negative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A. thaliana, as indicated by the
phenotypes. In addition, the expression of AtP5CS and AtRD29A, two genes tightly related to stress resistance, was
significantly lower in the overexpression lines than in the wild type. The growth of E. coli BL21 cells expressing
ScCaM showed weaker tolerance under mannitol and NaCl stress. Taken together, this study revealed that the ScCaM
gene plays a negative regulatory role in both mannitol and NaCl stresses, and it possibly exerts protective mechanisms
common in both prokaryotes and eukaryotes under stress conditions. |
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| Keywords: | Calmodulin (CaM) Signal transduction Drought Salt stress Sugarcane |
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