| 双水相体系萃取植物酯酶的研究 |
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| 引用本文： | 孟玲,杨丹,朱建星.双水相体系萃取植物酯酶的研究[J].沈阳化工学院学报,2006,20(4):254-257. |
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| 作者姓名： | 孟玲 杨丹 朱建星 |
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| 作者单位： | 1. 沈阳化工学院,辽宁,沈阳,110142;沈阳农业大学,辽宁,沈阳,110161
2. 沈阳化工学院,辽宁,沈阳,110142 |
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| 摘 要： | 研究植物酯酶在PEG1000胤酸铵双水相体系中的分配行为，探讨PEG、（NH4）2SO4用量以及pH等因素对植物酯酶和总蛋白分配系数、酶活回收率、相比的影响．实验表明当萃取体系中PEG1000、（NH4）2SO4的质量分数为77％和13％，pH值为7．5时。分配系数Kp,酶活回收率η和总蛋白的分配系数Kp分别为10．2462、94．8％和2．3160．
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| 关 键 词： | 双水相体系 植物酯酶 聚乙二醇 硫酸铵 |
| 文章编号： | 1004-4639（2006）04-0254-04 |
| 收稿时间： | 2006-03-22 |
| 修稿时间： | 2006-03-22 |
Extraction of Plant Esterase by Polyethylene Glycol Ammonium Sulfate Aqueous Two-phase System |
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| MENG Ling,YANG Dan,ZHU Jian-xing.Extraction of Plant Esterase by Polyethylene Glycol Ammonium Sulfate Aqueous Two-phase System[J].Journal of Shenyang Institute of Chemical Technolgy,2006,20(4):254-257. |
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| Authors: | MENG Ling YANG Dan ZHU Jian-xing |
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| Affiliation: | 1. Shenyang Institute of Chemical Technology, Shenyang 110142, China; 2. Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | The paper discussed the distribution of plant esterase in the PEG/(NH_4)_2SO_4 two-phase system.The dependence of partition coefficient of plant esterase and total protein,and the volume ratio of the top phase to the bottom phase on the percentage of PEG and(NH_4)_2SO_4,pH were studied respectively,and the appropriate separation conditions were determined.The suitable extraction conditions are 27 % PEG1000,13 %(NH_4)_2SO_4,pH 7.5.It was shown that the partition coefficient of K_(e) of (10.246 2),K_(p) of 2.316 0,the yield of 94.8 %. |
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| Keywords: | aqueous two-phase system plant esterase polyethylene glycol (NH_4)_2SO_4 |
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