活性黑5的生物脱色、复色现象及机理

印染废水问题形势依然严峻，目前常用生物法来治理，筛选高效降解染料的微生物是生物法处理印染废水的关键。本文利用梯度浓度压力驯化法，从印染废水水解酸化反应器中筛选出对染料活性黑5具有良好脱色性能的混合菌群DDMY1。利用该菌群在兼氧条件下对活性黑5进行脱色研究，首先采用拍照方式记录其反复脱色、复色的直观效果，其次运用紫外-可见光分光光度计检测其不同时间、不同状态下脱色液吸光值情况，最后运用气相色谱质谱联用仪和傅里叶变换红外光谱仪等检测方法分析脱色、复色过程中产物情况。结果表明，混合菌群DDMY1对活性黑5的脱色性能显著，24h脱色率能达到97.4%。同时，发现活性黑5的兼氧生物脱色反应可有效反复脱色、复色达17次之多，根据分析测试的结果，初步推测该现象可能是由活性黑5降解产物中的苯胺类物质或萘醌类物质造成的。

Decolorization and repigmentation of reactive black 5 biodegradation and their mechanisms

Wastewater from printing and dyeing industrials is still a severe problem,and biological treatment is the most widely used method currently, for which screening high efficient dye biodegradation microorganisms is the key. In this paper,by using the concentration gradient pressure screening method,we selected a mixed bacterial flora DDMY1,which has good decolorization performance to reactive black 5(RB5),from the well-running hydrolytic acidification reactor for subsequent printing and dyeing wastewater treatment. The bacterial flora was used to study the RB5 decolorization under the facultative-aerobic condition. Firstly,photos were taken in the process of decolorization and repigmentation to record the repeated results visually. Secondly,ultraviolet-visible spectrophotometry was used to scan the decoloring liquid at different times and different states. At last, GC-MS (Gas Chromatography-Mass Spectrometer) and FTIR (Fourier Transform Infrared Spectroscopy) were employed to analyze the degradation products. Results indicated that,the mixed flora DDMY1 performed satisfatorily in decoloring of RB5,and with 400mg/L RB5 for 24h cultivation,the decolorization rate could reach 97.4%. At the same time,the study found that the biological oxygen decolorization reaction could effectively repeat the decolorization-repigmentation for 17 times. According to the analysis results of the tests,it could be speculated that RB5 degradation products such as aniline,naphthoquinones might be responsible.