| 虫草属真菌的ITS序列分析和系统发育研究 |
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| 引用本文： | 刘小莉,周剑忠,王英,李剑,黄开红. 虫草属真菌的ITS序列分析和系统发育研究[J]. 现代食品科技, 2009, 25(5) |
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| 作者姓名： | 刘小莉 周剑忠 王英 李剑 黄开红 |
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| 作者单位： | 江苏省农业科学院农产品加工研究所,江苏,南京,210014;江苏省农业科学院农产品加工研究所,江苏,南京,210014;江苏省农业科学院农产品加工研究所,江苏,南京,210014;江苏省农业科学院农产品加工研究所,江苏,南京,210014;江苏省农业科学院农产品加工研究所,江苏,南京,210014 |
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| 基金项目： | 江苏省农业科学院创新基金 |
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| 摘 要： | 收集不同产地虫草属真菌,进行ITS(Internal Transcribed Spacer)区段克隆测序和序列特征比较分析,并对ITS序列进行核酸序列数据库GenBank同源性检索比对,并与检索获得的7个最相似物种的ITS序列构建系统发育树.结果表明,虫草属真菌的ITS序列长度为570～576bp,GC含量56.29～56.77%,系统发育分析显示,供试菌株中除C-6与九州虫草高度同源外,其他与蛹虫草均具有高度的亲缘关系.
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| 关 键 词： | 虫草 内转录间隔区 系统发育分析 |
ITS sequence analysis and phylogenetic study of Cordyceps spp. |
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| LIU Xiao-li,ZHOU Jian-zhong,WANG Ying,LI Jian,HUANG Kai-hong. ITS sequence analysis and phylogenetic study of Cordyceps spp.[J]. Modern Food Science & Technology, 2009, 25(5) |
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| Authors: | LIU Xiao-li ZHOU Jian-zhong WANG Ying LI Jian HUANG Kai-hong |
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| Affiliation: | Institute of Farm-product processing;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China |
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| Abstract: | In this paper, Cordyceps strains were collected from different regions of China and their sequences of internal transcribed spacers (ITS) were obtained and compared. Homology comparison was conducted between the obtained ITS sequences and reference sequences from GenBank database. Seven reference sequences were selected to construct the phylogenetic trees. Results showed that ITS sequences were 570-576bp, with the GC content of 56.29-56.77%. Phylogenetic analysis showed that, except for the strain C-6 simil... |
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| Keywords: | Cordyceps internal transcribed spacers phylogenetic analysis |
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