Organ-Specific Distributions of Lysophosphatidylcholine and Triacylglycerol in Mouse Embryo |
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Authors: | Takahiro Hayasaka Naoko Goto-Inoue Nobuhiro Zaima Yoshishige Kimura Mitsutoshi Setou |
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Affiliation: | (1) Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu Shizuoka, 431-3192, Japan; |
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Abstract: | Imaging mass spectrometry (IMS) has been developed as a method for determining and visualizing the distribution of proteins
and lipids across sections of dissected tissue. Although lipids play an important role in mammal development, their detailed
distributions have not been analyzed by conventional methods. In this study, we tried to determine and visualize lysophosphatidylcholine
(LysoPtdCho) and triacylglycerol (TAG) in a mouse embryo by matrix-assisted laser desorption/ionization (MALDI) hybrid quadrupole
time-of-flight (TOF) mass spectrometer. Many peaks were detected from a raster scan of the whole embryonic sections. The peaks
at m/z 496.33, 524.36, 879.72, 881.74, and 921.74 were identified by MS/MS analyses as LysoPtdCho (16:0) + H]+, LysoPtdCho (18:0) + H]+, TAG (16:0/18:2/18:1) + Na]+, TAG (16:0/18:1/18:1) + Na]+, and TAG (16:0/20:3/18:1) + K]+, respectively. The ion images constructed from the peaks revealed that LysoPtdCho were distributed throughout the body and
TAGs were distributed around the brown adipose tissue and in the liver at embryo day 17.5. Thus, IMS system based on MALDI
hybrid quadrupole TOF MS revealed the distribution of LysoPtdCho and, more importantly, the organ-specific distribution of
TAGs in the embryonic stages of mammals for the first time. We can conclude that this technique enables us to analyze the
roles of various lipids during embryogenesis and gives insight for lipid research. |
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