Method to produce 9(S)-hydroperoxides of linoleic and linolenic acids by maize lipoxygenase |
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Authors: | Harold W Gardner Marilyn J Grove |
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Affiliation: | (1) Mycotoxin Research, NCAUR, ARS, USDA, 1815 N. University St., 61604 Peoria, Illinois |
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Abstract: | Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)-hydroperoxy-10(F), 12(Z)-octadecadienoic acid and 9(S)-hydroperoxy-10(E), 12(Z), 15(Z)-octadecatrienoic acid, respectively. Corn seed has a very active hydro-peroxide-decomposing enzyme, allene oxide synthase
(AOS), which must be removed prior to oxidizing the fatty acid. A simple pH 4.5 treatment followed by centrifugation removes
most of the AOS activity. Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement
in 9-hydroperoxide formation. This facile alternative method of preparing 9-hydroperoxides has advantages over other commonly
used plant lipoxygenases. |
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